TY - JOUR
T1 - Sex-conversion from male to female during somatic embryogenesis from protoplasts in asparagus (Asparagus officinalis L.)
AU - Maeda, Tomoo
AU - Ozaki, Yukio
AU - Sonoda, Takahiro
AU - Inoue, Nobuhisa
AU - Narikiyo, Kumiko
AU - Okubo, Hiroshi
PY - 2005/10
Y1 - 2005/10
N2 - Sex-converting mutation from male to female was identified during somatic embryogenesis from protoplasts in asparagus. Although modification of allozyme genotypes from heterozygous to homozygous occurred in three loci, Mdh-1, Mdh-2 and Idh-1, accompanied with sex-conversion, genotypes of sex-converted and control plants in Pgm-1 and Skdh-1 loci were identically heterozygous. Relative DNA contents of sex-converted and control plants estimated by flow cytometric analysis were similar, and gametophytic fertility of the sex-converting mutant as female parent was also confirmed by artificial pollination. From these results, the sex-conversion in the current study might be the result of the somatic mutation, such as somatic crossing-over, one of the chromosomal rearrangements.
AB - Sex-converting mutation from male to female was identified during somatic embryogenesis from protoplasts in asparagus. Although modification of allozyme genotypes from heterozygous to homozygous occurred in three loci, Mdh-1, Mdh-2 and Idh-1, accompanied with sex-conversion, genotypes of sex-converted and control plants in Pgm-1 and Skdh-1 loci were identically heterozygous. Relative DNA contents of sex-converted and control plants estimated by flow cytometric analysis were similar, and gametophytic fertility of the sex-converting mutant as female parent was also confirmed by artificial pollination. From these results, the sex-conversion in the current study might be the result of the somatic mutation, such as somatic crossing-over, one of the chromosomal rearrangements.
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U2 - 10.5109/4671
DO - 10.5109/4671
M3 - Article
AN - SCOPUS:27744482337
SN - 0023-6152
VL - 50
SP - 585
EP - 592
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
IS - 2
ER -