Lentinula edodes was cultivated in the artificial blocks of sawdust, and the fruiting body was induced on the surface of the sawdust blocks. The four different fungal stages; mycelia (D), primordia (C), stipes (B), and pileus (A) of fruiting bodies were collected for the extraction of mRNA, searching the genes related to morphogenesis. The three fragments of cDNA not showed in the stage (A) were picked up and displayed, to be two bands in the agarose gels. Total five bands were selected for analyzing the DNA sequences, using the pRIP-T™ vector system. The fragments of cDNA investigated in this work were not those related to the structural genes (exons), but to be related to the controlling genes (introns).
|Number of pages||13|
|Journal||Journal of the Faculty of Agriculture, Kyushu University|
|Publication status||Published - Oct 2001|
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science