Several genes expression on fructification of Lentinula edodes

Sang Sun Lee; Shoji Ohga

doi:10.5109/24421

Several genes expression on fructification of Lentinula edodes

Sang Sun Lee, Shoji Ohga

University Forest

Research output: Contribution to journal › Article › peer-review

Abstract

Lentinula edodes was cultivated in the artificial blocks of sawdust, and the fruiting body was induced on the surface of the sawdust blocks. The four different fungal stages; mycelia (D), primordia (C), stipes (B), and pileus (A) of fruiting bodies were collected for the extraction of mRNA, searching the genes related to morphogenesis. The three fragments of cDNA not showed in the stage (A) were picked up and displayed, to be two bands in the agarose gels. Total five bands were selected for analyzing the DNA sequences, using the pRIP-T™ vector system. The fragments of cDNA investigated in this work were not those related to the structural genes (exons), but to be related to the controlling genes (introns).

Original language	English
Pages (from-to)	47-59
Number of pages	13
Journal	Journal of the Faculty of Agriculture, Kyushu University
Volume	46
Issue number	1
DOIs	https://doi.org/10.5109/24421
Publication status	Published - Oct 2001

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Biotechnology

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abstract = "Lentinula edodes was cultivated in the artificial blocks of sawdust, and the fruiting body was induced on the surface of the sawdust blocks. The four different fungal stages; mycelia (D), primordia (C), stipes (B), and pileus (A) of fruiting bodies were collected for the extraction of mRNA, searching the genes related to morphogenesis. The three fragments of cDNA not showed in the stage (A) were picked up and displayed, to be two bands in the agarose gels. Total five bands were selected for analyzing the DNA sequences, using the pRIP-T{\texttrademark} vector system. The fragments of cDNA investigated in this work were not those related to the structural genes (exons), but to be related to the controlling genes (introns).",

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AB - Lentinula edodes was cultivated in the artificial blocks of sawdust, and the fruiting body was induced on the surface of the sawdust blocks. The four different fungal stages; mycelia (D), primordia (C), stipes (B), and pileus (A) of fruiting bodies were collected for the extraction of mRNA, searching the genes related to morphogenesis. The three fragments of cDNA not showed in the stage (A) were picked up and displayed, to be two bands in the agarose gels. Total five bands were selected for analyzing the DNA sequences, using the pRIP-T™ vector system. The fragments of cDNA investigated in this work were not those related to the structural genes (exons), but to be related to the controlling genes (introns).

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Several genes expression on fructification of Lentinula edodes

Abstract

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