TY - JOUR
T1 - Serum vitamin B6, folate, and homocysteine concentrations and oxidative DNA damage in Japanese men and women
AU - Kuwahara, Keisuke
AU - Nanri, Akiko
AU - Pham, Ngoc Minh
AU - Kurotani, Kayo
AU - Kume, Ayami
AU - Sato, Masao
AU - Kawai, Kazuaki
AU - Kasai, Hiroshi
AU - Mizoue, Tetsuya
N1 - Funding Information:
We thank Tamami Hatano, Yasumi Kimura, Akihiro Tanaka, and Yuko Ejima (Kyushu University); Mio Ozawa (Fukuoka Women's University); Yaeko Nagano (retired nurse); and Akiko Hayashi, and Kie Nagao (National Center for Global Health and Medicine) for their help in data collection. This study was supported by Grant-in-Aid for Young Scientists (B)( 21790598 ) from the Ministry of Education, Culture, Sports, Science and Technology (to Dr Nanri) and Grant-in-Aid for Scientific Research (C)( 18590601 ) and (B)( 21390213 ) from Japan Society for the Promotion of Science (to Dr Mizoue).
PY - 2013/10
Y1 - 2013/10
N2 - Objective: Higher vitamin B status has been linked to a lower risk for cancer, but the underlying mechanism remains elusive. The aim of the present study was to examine the association of pyridoxal, folate, and homocysteine (Hcy) with urinary 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative DNA damage. Methods: The participants were 500 employees (293 men and 207 women), ages 21 to 66 y, of two municipal offices in Japan. Serum pyridoxal and Hcy concentrations were measured using high-performance liquid chromatography (HPLC) method, and serum folate concentrations were measured using chemiluminescent immunoassay. Urinary 8-OHdG concentrations were measured using HPLC method. Multiple regression was used to estimate means of 8-OHdG for each tertile of pyridoxal, folate, and Hcy with adjustment for potential confounders. Results: In multivariate analysis, 8-OHdG concentration was inversely associated with pyridoxal concentration in men (P for trend = 0.045) but not in women. The association in men was confined to non-smokers (P for trend = 0.033) or those who consumed no or < 20 g/d of ethanol (P for trend = 0.048). 8-OHdG concentrations were not appreciably associated with folate and Hcy concentrations. Conclusion: The results suggest that vitamin B6, but not folate and homocysteine, plays a role against oxidative DNA damage in Japanese men.
AB - Objective: Higher vitamin B status has been linked to a lower risk for cancer, but the underlying mechanism remains elusive. The aim of the present study was to examine the association of pyridoxal, folate, and homocysteine (Hcy) with urinary 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative DNA damage. Methods: The participants were 500 employees (293 men and 207 women), ages 21 to 66 y, of two municipal offices in Japan. Serum pyridoxal and Hcy concentrations were measured using high-performance liquid chromatography (HPLC) method, and serum folate concentrations were measured using chemiluminescent immunoassay. Urinary 8-OHdG concentrations were measured using HPLC method. Multiple regression was used to estimate means of 8-OHdG for each tertile of pyridoxal, folate, and Hcy with adjustment for potential confounders. Results: In multivariate analysis, 8-OHdG concentration was inversely associated with pyridoxal concentration in men (P for trend = 0.045) but not in women. The association in men was confined to non-smokers (P for trend = 0.033) or those who consumed no or < 20 g/d of ethanol (P for trend = 0.048). 8-OHdG concentrations were not appreciably associated with folate and Hcy concentrations. Conclusion: The results suggest that vitamin B6, but not folate and homocysteine, plays a role against oxidative DNA damage in Japanese men.
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U2 - 10.1016/j.nut.2013.03.014
DO - 10.1016/j.nut.2013.03.014
M3 - Article
C2 - 23800563
AN - SCOPUS:84884206285
SN - 0899-9007
VL - 29
SP - 1219
EP - 1223
JO - Nutrition
JF - Nutrition
IS - 10
ER -
