Serum vitamin B6, folate, and homocysteine concentrations and oxidative DNA damage in Japanese men and women

Keisuke Kuwahara, Akiko Nanri, Ngoc Minh Pham, Kayo Kurotani, Ayami Kume, Masao Sato, Kazuaki Kawai, Hiroshi Kasai, Tetsuya Mizoue

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Objective: Higher vitamin B status has been linked to a lower risk for cancer, but the underlying mechanism remains elusive. The aim of the present study was to examine the association of pyridoxal, folate, and homocysteine (Hcy) with urinary 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative DNA damage. Methods: The participants were 500 employees (293 men and 207 women), ages 21 to 66 y, of two municipal offices in Japan. Serum pyridoxal and Hcy concentrations were measured using high-performance liquid chromatography (HPLC) method, and serum folate concentrations were measured using chemiluminescent immunoassay. Urinary 8-OHdG concentrations were measured using HPLC method. Multiple regression was used to estimate means of 8-OHdG for each tertile of pyridoxal, folate, and Hcy with adjustment for potential confounders. Results: In multivariate analysis, 8-OHdG concentration was inversely associated with pyridoxal concentration in men (P for trend = 0.045) but not in women. The association in men was confined to non-smokers (P for trend = 0.033) or those who consumed no or < 20 g/d of ethanol (P for trend = 0.048). 8-OHdG concentrations were not appreciably associated with folate and Hcy concentrations. Conclusion: The results suggest that vitamin B6, but not folate and homocysteine, plays a role against oxidative DNA damage in Japanese men.

Original languageEnglish
Pages (from-to)1219-1223
Number of pages5
Issue number10
Publication statusPublished - Oct 2013

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics


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