TY - JOUR
T1 - Serum choline plasmalogens-those with oleic acid in sn-2-are biomarkers for coronary artery disease
AU - Nishimukai, Megumi
AU - Maeba, Ryouta
AU - Ikuta, Akiko
AU - Asakawa, Naoya
AU - Kamiya, Kiwamu
AU - Yamada, Shiro
AU - Yokota, Takashi
AU - Sakakibara, Mamoru
AU - Tsutsui, Hiroyuki
AU - Sakurai, Toshihiro
AU - Takahashi, Yuji
AU - Hui, Shu Ping
AU - Chiba, Hitoshi
AU - Okazaki, Tomoki
AU - Hara, Hiroshi
N1 - Funding Information:
This study was supported by the Knowledge Cluster Initiative (2nd stage, Sapporo Biocluster Bio-S) of the Ministry of Education, Science, Sports and Culture of Japan .
Publisher Copyright:
© 2014 Elsevier B.V..
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Background: Identifying risk factors is crucial for preventing cardiovascular events, but there are no widely accepted predictive biomarkers. In our previous study of Japanese asymptomatic cohorts, we performed global analysis of serum ether glycerophospholipids (Egp) molecular profiles, and found that choline plasmalogens (PlsCho; 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphocholine), particularly those containing oleic acid (18:1) in the sn-2 position, were strongly associated with a wide range of risk factors for metabolic syndrome/atherosclerosis. Methods: We determined serum concentrations of Egp molecular species of coronary artery disease patients (n = 50; 31 males and 19 females) by LC/MS/MS, and plasmalogen (Pls; 1-O-alk-1'-enyl-2-acyl-sn-glycerophospholipids) contents in lipoprotein fractions by HPLC using radioactive iodine. Results: We found that the serum concentrations of ether choline glycerophospholipids (EgpCho), particularly PlsCho, were not only significantly lower in males with significant coronary stenosis but also associated with atherosclerosis-related parameters, and their association was stronger than either high-density lipoprotein cholesterol or adiponectin. In addition, serum PlsCho containing 18:1 or linoleic acid (18:2) in sn-2 showed the highest correlations with a wide range of atherogenic parameters among PlsCho molecular species. Conclusion: These results verify our previous findings that serum PlsCho, particularly those containing 18:1 in sn-2, may serve as reliable biomarkers for atherosclerosis.
AB - Background: Identifying risk factors is crucial for preventing cardiovascular events, but there are no widely accepted predictive biomarkers. In our previous study of Japanese asymptomatic cohorts, we performed global analysis of serum ether glycerophospholipids (Egp) molecular profiles, and found that choline plasmalogens (PlsCho; 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphocholine), particularly those containing oleic acid (18:1) in the sn-2 position, were strongly associated with a wide range of risk factors for metabolic syndrome/atherosclerosis. Methods: We determined serum concentrations of Egp molecular species of coronary artery disease patients (n = 50; 31 males and 19 females) by LC/MS/MS, and plasmalogen (Pls; 1-O-alk-1'-enyl-2-acyl-sn-glycerophospholipids) contents in lipoprotein fractions by HPLC using radioactive iodine. Results: We found that the serum concentrations of ether choline glycerophospholipids (EgpCho), particularly PlsCho, were not only significantly lower in males with significant coronary stenosis but also associated with atherosclerosis-related parameters, and their association was stronger than either high-density lipoprotein cholesterol or adiponectin. In addition, serum PlsCho containing 18:1 or linoleic acid (18:2) in sn-2 showed the highest correlations with a wide range of atherogenic parameters among PlsCho molecular species. Conclusion: These results verify our previous findings that serum PlsCho, particularly those containing 18:1 in sn-2, may serve as reliable biomarkers for atherosclerosis.
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U2 - 10.1016/j.cca.2014.07.024
DO - 10.1016/j.cca.2014.07.024
M3 - Article
C2 - 25068205
AN - SCOPUS:84910059075
SN - 0009-8981
VL - 437
SP - 147
EP - 154
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -