Sequential injection immunoassay for environmental measurements

Nobuaki Soh, Mayumi Tanaka, Koji Hirakawa, Rui Qi Zhang, Hizuru Nakajima, Koji Nakano, Toshihiko Imato

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)


    Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.

    Original languageEnglish
    Pages (from-to)1069-1076
    Number of pages8
    Journalanalytical sciences
    Issue number11
    Publication statusPublished - 2011

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry


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