Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

Mitsuhiko Onda; Yuri Lvov; Katsuhiko Ariga; Toyoki Kunitake

doi:10.1002/(SICI)1097-0290(19960720)51:2<163::AID-BIT5>3.0.CO;2-H

Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

Mitsuhiko Onda, Yuri Lvov, Katsuhiko Ariga, Toyoki Kunitake

Research output: Contribution to journal › Article › peer-review

265 Citations (Scopus)

Abstract

Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H₂O₂) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEI/GOD. Sequential redox reaction of glucose/H₂O₂/DA67 was demonstrated successfully with this supramolecular system.

Original language	English
Pages (from-to)	163-167
Number of pages	5
Journal	Biotechnology and Bioengineering
Volume	51
Issue number	2
DOIs	https://doi.org/10.1002/(SICI)1097-0290(19960720)51:2<163::AID-BIT5>3.0.CO;2-H
Publication status	Published - Jul 20 1996
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/(SICI)1097-0290(19960720)51:2<163::AID-BIT5>3.0.CO;2-H

Cite this

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title = "Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption",

abstract = "Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H2O2) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEI/GOD. Sequential redox reaction of glucose/H2O2/DA67 was demonstrated successfully with this supramolecular system.",

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T1 - Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

AU - Onda, Mitsuhiko

AU - Lvov, Yuri

AU - Ariga, Katsuhiko

AU - Kunitake, Toyoki

PY - 1996/7/20

Y1 - 1996/7/20

N2 - Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H2O2) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEI/GOD. Sequential redox reaction of glucose/H2O2/DA67 was demonstrated successfully with this supramolecular system.

AB - Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H2O2) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEI/GOD. Sequential redox reaction of glucose/H2O2/DA67 was demonstrated successfully with this supramolecular system.

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Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

Abstract

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