TY - JOUR
T1 - Sequence motif in control regions of the H+/K+ ATPase α and β subunit genes recognized by gastric specific nuclear protein(s)
AU - Tamura, Shigehiko
AU - Oshiman, Ko Ichi
AU - Nishi, Tsuyoshi
AU - Mori, Masaharu
AU - Maeda, Masatomo
AU - Futai, Masamitsu
N1 - Funding Information:
,4c~rtowle&enreTnhrsis: workw ass upportedb yg rantsf romt heM in-istryo f EducarionS, ciencea nd Cultureo f Japan,a HumanF rontier ScienceP rogram ResearchG rant, and a grant from the Naito FoundationW. e thankD r. K. Sekimizu(T okyoU niversity)f or hclp-ful discussions.
PY - 1992/2/24
Y1 - 1992/2/24
N2 - A nuclear protein(s) from rat or pig stomach recognized a conserved sequence in the 5′-upstream regions of the rat and human H+/K+-ATPase α subunit genes. A gel retardation assay suggested that part of the binding site was located in the TAATCAGCTG sequence. No nuclear proteins capable of the binding could be detected in other tissues of rat (liver, brain, kidney, spleen and lung) or pig liver. The sequence motif (GATAGC) located 5′-upstream of the β-subunit gene also seemed to be recognized by the same protein, because the binding of nuclear protein to the sequence motifs in the α and β subunits was mutually competitive. Considering the sense-strand sequence of the binding motif in the α-subunit gene, we conclude that (G/C)PuPu(G/C)NGAT(A/T)PuPy is a core sequence motif for the gastric specific DNA binding protein (PCSF, parietal cell specific factor).
AB - A nuclear protein(s) from rat or pig stomach recognized a conserved sequence in the 5′-upstream regions of the rat and human H+/K+-ATPase α subunit genes. A gel retardation assay suggested that part of the binding site was located in the TAATCAGCTG sequence. No nuclear proteins capable of the binding could be detected in other tissues of rat (liver, brain, kidney, spleen and lung) or pig liver. The sequence motif (GATAGC) located 5′-upstream of the β-subunit gene also seemed to be recognized by the same protein, because the binding of nuclear protein to the sequence motifs in the α and β subunits was mutually competitive. Considering the sense-strand sequence of the binding motif in the α-subunit gene, we conclude that (G/C)PuPu(G/C)NGAT(A/T)PuPy is a core sequence motif for the gastric specific DNA binding protein (PCSF, parietal cell specific factor).
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U2 - 10.1016/0014-5793(92)80040-N
DO - 10.1016/0014-5793(92)80040-N
M3 - Article
C2 - 1312019
AN - SCOPUS:0026536740
SN - 0014-5793
VL - 298
SP - 137
EP - 141
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -