Separation of polyprenol and dolichol by monolithic silica capillary column chromatography

Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio Kobayashi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


We attempted an analysis of naturally occurring polyprenol and dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mixture alone was performed using a 250 × 0.2 mm inner diameter (ID) octadecylsilyl (ODS)-monolithic silica capillary column. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by ≥ 2-fold the level recorded when using a conventional ODS-silica particle-packed column (250 × 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and dolichol type (dihydropolyprenol) was subjected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analysis of polyprenol fraction derived from Eucommia ulmoides leaves, dolichols were found as a single peak, including all-trans-polyprenol and cis-polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polyprenols and dolichols and that have a low content.

Original languageEnglish
Pages (from-to)2295-2298
Number of pages4
JournalJournal of Lipid Research
Issue number10
Publication statusPublished - Oct 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology


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