TY - JOUR
T1 - Separated transcriptomes of male gametophyte and tapetum in rice
T2 - Validity of a laser microdissection (LM) microarray
AU - Suwabe, Keita
AU - Suzuki, Go
AU - Takahashi, Hirokazu
AU - Shiono, Katsuhiro
AU - Endo, Makoto
AU - Yano, Kentaro
AU - Fujita, Masahiro
AU - Masuko, Hiromi
AU - Saito, Hiroshi
AU - Fujioka, Tomoaki
AU - Kaneko, Fumi
AU - Kazama, Tomohiko
AU - Mizuta, Yoko
AU - Kawagishi-Kobayashi, Makiko
AU - Tsutsumi, Nobuhiro
AU - Kurata, Nori
AU - Nakazono, Mikio
AU - Watanabe, Masao
N1 - Funding Information:
The Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT), Grants-in-Aid for Special Research on Priority Areas (Nos. 18075003 and 18075012 to M.W., 18075005 to N.T., 18075013 to N.K., 19043015 to K.Y.); the Japan Society for Promotion of Science (JSPS) Grants-in-Aid for the 21st Century Center of Excellence Program (to M.W.); the Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF) grant for the Integrated Research Project for Plant, Insect, and Animal using Genome Technology (Nos. IPG-0012 to M.N., IPG-0018 to M.K.K., IPG-0019 to M.W.); Research Fellowship for Postdoctoral Fellowships for Young Researchers of JSPS (K.S.).
PY - 2008/10
Y1 - 2008/10
N2 - In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.
AB - In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.
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U2 - 10.1093/pcp/pcn124
DO - 10.1093/pcp/pcn124
M3 - Article
C2 - 18755754
AN - SCOPUS:54149088703
SN - 0032-0781
VL - 49
SP - 1407
EP - 1416
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 10
ER -