TY - JOUR
T1 - Sensitive determination of alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine using gas chromatography-mass spectrometry
AU - Ishida, Tomomi
AU - Kudo, Keiko
AU - Kiyoshima, Akiko
AU - Inoue, Hiromasa
AU - Tsuji, Akiko
AU - Ikeda, Noriaki
N1 - Funding Information:
This work was supported by a Research Foundation for Safe Society and Grant-in-Aid for Scientific Research (No. 16390194) from Ministry of Education, Science, Sports and Culture. We thank Dr. T. Kojima of Kanagawa Prefectural Institute of Public Health for his helpful comments and M. Ohara (Fukuoka, Japan) for language assistance.
PY - 2005/8/25
Y1 - 2005/8/25
N2 - We devised a sensitive and simple method to determine alpha- methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine, using gas chromatography-mass spectrometry (GC-MS). AMT and 5MeO-DIPT were extracted using an Extrelut® column with an internal standard, bupivacaine, followed by derivatization with acetic anhydride. The derivatized extract was used for GC-MS analysis of EI-SIM mode. The calibration curves of AMT and 5MeO-DIPT were linear in the concentration range from 10 to 750 ng/ml in both blood and urine samples. The method detection limit (MDL) of AMT and 5MeO-DIPT were 1 ng/ml each in whole blood and 5 ng/ml each in urine. This method should be most useful to accurately determine the presence of these drugs in blood and urine in clinical and forensic cases.
AB - We devised a sensitive and simple method to determine alpha- methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine, using gas chromatography-mass spectrometry (GC-MS). AMT and 5MeO-DIPT were extracted using an Extrelut® column with an internal standard, bupivacaine, followed by derivatization with acetic anhydride. The derivatized extract was used for GC-MS analysis of EI-SIM mode. The calibration curves of AMT and 5MeO-DIPT were linear in the concentration range from 10 to 750 ng/ml in both blood and urine samples. The method detection limit (MDL) of AMT and 5MeO-DIPT were 1 ng/ml each in whole blood and 5 ng/ml each in urine. This method should be most useful to accurately determine the presence of these drugs in blood and urine in clinical and forensic cases.
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U2 - 10.1016/j.jchromb.2004.10.067
DO - 10.1016/j.jchromb.2004.10.067
M3 - Article
C2 - 16055053
AN - SCOPUS:23044496715
SN - 1570-0232
VL - 823
SP - 47
EP - 52
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -