TY - JOUR
T1 - SEN1 gene from Lotus japonicus MG20 improves nitrogen fixation and plant growth
AU - Nishida, Yuki
AU - Hiraoka, Reona
AU - Kawano, Satomi
AU - Suganuma, Norio
AU - Sato, Shusei
AU - Watanabe, Satoshi
AU - Anai, Toyoaki
AU - Arima, Susumu
AU - Tominaga, Akiyoshi
AU - Suzuki, Akihiro
N1 - Funding Information:
The seeds of Lotus japonicus Miyakojima MG20, Gifu B129, and RI-088 were kindly provided by The National BioResource Project Lotus japonicus and Glycine max. Critical comments of the editor and anonymous reviewers helped the authors to improve the manuscript. This work was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research Grant Number (B) 25292014.
Funding Information:
This work was supported by the Japan Society for the Promotion of Science [Grant-in-aid for scientific research (B) 25292014]. The seeds of Lotus japonicus Miyakojima MG20, Gifu B129, and RI-088 were kindly provided by The National BioResource Project Lotus japonicus and Glycine max. Critical comments of the editor and anonymous reviewers helped the authors to improve the manuscript. This work was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research Grant Number (B) 25292014.
Publisher Copyright:
© 2020 Japanese Society of Soil Science and Plant Nutrition.
PY - 2020
Y1 - 2020
N2 - In Lotus japonicus, quantitative trait loci (QTLs) for nitrogen fixation activity (acetylene reduction activity, ARA) and seed weight are located in the same region on chromosome 4 as the gene responsible for the Fix− mutant sen1. The SEN1 gene of L. japonicus Gifu B129 has a deletion of three nucleotides in comparison with that of L. japonicus Miyakojima MG20, which results in a deletion of one amino acid. Here, we examined whether SEN1 is responsible for these QTLs. Because the MG20 allele of the QTL for ARA and seed weight has a positive effect, we generated a near-isogenic line (NIL) in which the SEN1 gene region of B129 was replaced with that of MG20. Growth parameters of this NIL (MG20-type) 21 days after inoculation with Mesorhizobium loti were significantly higher than those of B129-type, and ARA was also significantly higher. Nodules formed on hairy roots in which the sen1 mutant was complemented with the MG20 SEN1 gene had higher ARA than those of B129. The seeds of the MG20-type NIL were significantly heavier than those of B129-type. These results indicate that the SEN1 gene is a very likely candidate for the QTL for ARA and seed weight on chromosome 4.
AB - In Lotus japonicus, quantitative trait loci (QTLs) for nitrogen fixation activity (acetylene reduction activity, ARA) and seed weight are located in the same region on chromosome 4 as the gene responsible for the Fix− mutant sen1. The SEN1 gene of L. japonicus Gifu B129 has a deletion of three nucleotides in comparison with that of L. japonicus Miyakojima MG20, which results in a deletion of one amino acid. Here, we examined whether SEN1 is responsible for these QTLs. Because the MG20 allele of the QTL for ARA and seed weight has a positive effect, we generated a near-isogenic line (NIL) in which the SEN1 gene region of B129 was replaced with that of MG20. Growth parameters of this NIL (MG20-type) 21 days after inoculation with Mesorhizobium loti were significantly higher than those of B129-type, and ARA was also significantly higher. Nodules formed on hairy roots in which the sen1 mutant was complemented with the MG20 SEN1 gene had higher ARA than those of B129. The seeds of the MG20-type NIL were significantly heavier than those of B129-type. These results indicate that the SEN1 gene is a very likely candidate for the QTL for ARA and seed weight on chromosome 4.
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U2 - 10.1080/00380768.2020.1834829
DO - 10.1080/00380768.2020.1834829
M3 - Article
AN - SCOPUS:85094881266
SN - 0038-0768
VL - 66
SP - 864
EP - 869
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
IS - 6
ER -