TY - JOUR
T1 - Self-regulated left-right asymmetric expression of Pitx2c in the developing mouse limb
AU - Shiratori, Hidetaka
AU - Yashiro, Kenta
AU - Iwai, Naomi
AU - Oki, Shinya
AU - Minegishi, Katsura
AU - Ikawa, Yayoi
AU - Kanata, Kohei
AU - Hamada, Hiroshi
N1 - Funding Information:
We thank A. Fukumoto, Y. Ikawa, and H. Nishimura for technical assistance. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan ; by Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Corporation (JST).
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/11/15
Y1 - 2014/11/15
N2 - The transcription factor Pitx2c is expressed in primordial visceral organs in a left-right (L-R) asymmetric manner and executes situs-specific morphogenesis. Here we show that Pitx2c is also L-R asymmetrically expressed in the developing mouse limb. Human PITX2c exhibits the same transcriptional activity in the mouse limb. The asymmetric expression of Pitx2c in the limb also exhibits dorsal-ventral and anterior-posterior polarities, being confined to the posterior-dorsal region of the left limb. Left-sided Pitx2c expression in the limb is regulated by Nodal signaling through a Nodal-responsive enhancer. Pitx2c is expressed in lateral plate mesoderm (LPM)-derived cells in the left limb that contribute to various limb connective tissues. The number of Pitx2c+ cells in the left limb was found to be negatively regulated by Pitx2c itself. Although obvious defects were not apparent in the limb of mice lacking asymmetric Pitx2c expression, Pitx2c may regulate functional L-R asymmetry of the limb.
AB - The transcription factor Pitx2c is expressed in primordial visceral organs in a left-right (L-R) asymmetric manner and executes situs-specific morphogenesis. Here we show that Pitx2c is also L-R asymmetrically expressed in the developing mouse limb. Human PITX2c exhibits the same transcriptional activity in the mouse limb. The asymmetric expression of Pitx2c in the limb also exhibits dorsal-ventral and anterior-posterior polarities, being confined to the posterior-dorsal region of the left limb. Left-sided Pitx2c expression in the limb is regulated by Nodal signaling through a Nodal-responsive enhancer. Pitx2c is expressed in lateral plate mesoderm (LPM)-derived cells in the left limb that contribute to various limb connective tissues. The number of Pitx2c+ cells in the left limb was found to be negatively regulated by Pitx2c itself. Although obvious defects were not apparent in the limb of mice lacking asymmetric Pitx2c expression, Pitx2c may regulate functional L-R asymmetry of the limb.
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U2 - 10.1016/j.ydbio.2014.09.002
DO - 10.1016/j.ydbio.2014.09.002
M3 - Article
C2 - 25224222
AN - SCOPUS:84908143897
SN - 0012-1606
VL - 395
SP - 331
EP - 341
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -