TY - JOUR
T1 - Selective fluorescence detection of histamine based on ligand exchange mechanism and its application to biomonitoring
AU - Seto, Daisuke
AU - Soh, Nobuaki
AU - Nakano, Koji
AU - Imato, Toshihiko
PY - 2010/9
Y1 - 2010/9
N2 - We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+ ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented.
AB - We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+ ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented.
UR - http://www.scopus.com/inward/record.url?scp=77954457315&partnerID=8YFLogxK
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U2 - 10.1016/j.ab.2010.04.038
DO - 10.1016/j.ab.2010.04.038
M3 - Article
C2 - 20450876
AN - SCOPUS:77954457315
SN - 0003-2697
VL - 404
SP - 135
EP - 139
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -