Selective Extraction of Active α-Chymotrypsin by Reversed Micelles

Masahiro Goto, Maki Korenaga, Tsutomu Ono, Shintaro Furusaki

    Research output: Contribution to journalArticlepeer-review


    Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed. Key words: extraction, reverse micelle, surfactant, protein separation, AOT.

    Original languageEnglish
    Pages (from-to)181-185
    Number of pages5
    Journalkagaku kogaku ronbunshu
    Issue number2
    Publication statusPublished - 2001

    All Science Journal Classification (ASJC) codes

    • General Chemistry
    • General Chemical Engineering


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