TY - JOUR
T1 - Schizosaccharomyces pombe UDP-galactose transporter
T2 - Identification of its functional form through cDNA cloning and expression in mammalian cells
AU - Segawa, Hiroaki
AU - Ishida, Nobuhiro
AU - Takegawa, Kaoru
AU - Kawakita, Masao
N1 - Funding Information:
We are grateful to Dr. K. Maruyama for providing us with pMKIT-neo plasmid. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Area No. 10178220 from the Ministry of Education, Science, Sports and Culture of Japan and by a grant from Kirin Brewery Co., Japan.
PY - 1999/5/28
Y1 - 1999/5/28
N2 - The Schizosaccharomyces pombe UDP-galactose transporter cDNA (SpUGT cDNA), encoding the product of the gms1+ gene which consists of two exon sequences separated by a 173-bp intron, was cloned by RT-PCR. Its product, a hydrophobic protein of 353 amino acid residues resembling its human counterpart, was expressed in the Golgi membranes of UDP-galactose transporter-deficient Lec8 cells, and complemented the genetic defect of the mutant cells. This indicated that SpUGT cDNA encodes the functional S. pombe UDP-galactose transporter. The product of an ORF found in the second exon, which was previously assumed to be the S. pombe UDP-galactose transporter, thus represents an inactive, truncated form of the SpUGT protein. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - The Schizosaccharomyces pombe UDP-galactose transporter cDNA (SpUGT cDNA), encoding the product of the gms1+ gene which consists of two exon sequences separated by a 173-bp intron, was cloned by RT-PCR. Its product, a hydrophobic protein of 353 amino acid residues resembling its human counterpart, was expressed in the Golgi membranes of UDP-galactose transporter-deficient Lec8 cells, and complemented the genetic defect of the mutant cells. This indicated that SpUGT cDNA encodes the functional S. pombe UDP-galactose transporter. The product of an ORF found in the second exon, which was previously assumed to be the S. pombe UDP-galactose transporter, thus represents an inactive, truncated form of the SpUGT protein. Copyright (C) 1999 Federation of European Biochemical Societies.
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U2 - 10.1016/S0014-5793(99)00596-7
DO - 10.1016/S0014-5793(99)00596-7
M3 - Article
C2 - 10371208
AN - SCOPUS:0032981386
SN - 0014-5793
VL - 451
SP - 295
EP - 298
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -