Saturated two-photon excitation fluorescence microscopy with core-ring illumination

Ryosuke Oketani; Atsushi Doi; Nicholas I. Smith; Yasunori Nawa; Satoshi Kawata; Katsumasa Fujita

doi:10.1364/OL.42.000571

Saturated two-photon excitation fluorescence microscopy with core-ring illumination

Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

We demonstrated resolution improvement in two-photon excitation microscopy by combining saturated excitation (SAX) of fluorescence and pupil manipulation. We theoretically estimated the resolution improvement and the sidelobe effect in the point spread function with various pupil designs and found that the combination of SAX and core-ring illumination can effectively enhance the spatial resolution in 3D and suppress sidelobe artifacts. The experimental demonstration shows that the proposed technique is effective for observation with a depth of 100 μm in a tissue phantom and can be applied to 3D observations of tissue samples with higher spatial resolution than conventional two-photon excitation microscopy.

Original language	English
Pages (from-to)	571-574
Number of pages	4
Journal	Optics Letters
Volume	42
Issue number	3
DOIs	https://doi.org/10.1364/OL.42.000571
Publication status	Published - Feb 1 2017
Externally published	Yes

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OL.42.000571

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title = "Saturated two-photon excitation fluorescence microscopy with core-ring illumination",

abstract = "We demonstrated resolution improvement in two-photon excitation microscopy by combining saturated excitation (SAX) of fluorescence and pupil manipulation. We theoretically estimated the resolution improvement and the sidelobe effect in the point spread function with various pupil designs and found that the combination of SAX and core-ring illumination can effectively enhance the spatial resolution in 3D and suppress sidelobe artifacts. The experimental demonstration shows that the proposed technique is effective for observation with a depth of 100 μm in a tissue phantom and can be applied to 3D observations of tissue samples with higher spatial resolution than conventional two-photon excitation microscopy.",

author = "Ryosuke Oketani and Atsushi Doi and Smith, {Nicholas I.} and Yasunori Nawa and Satoshi Kawata and Katsumasa Fujita",

note = "Funding Information: Core Research for Evolutional Science and Technology (CREST); Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) (KAKENHI 15J01780). Publisher Copyright: {\textcopyright} 2017 Optical Society of America.",

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T1 - Saturated two-photon excitation fluorescence microscopy with core-ring illumination

AU - Oketani, Ryosuke

AU - Doi, Atsushi

AU - Smith, Nicholas I.

AU - Nawa, Yasunori

AU - Kawata, Satoshi

AU - Fujita, Katsumasa

N1 - Funding Information: Core Research for Evolutional Science and Technology (CREST); Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) (KAKENHI 15J01780). Publisher Copyright: © 2017 Optical Society of America.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - We demonstrated resolution improvement in two-photon excitation microscopy by combining saturated excitation (SAX) of fluorescence and pupil manipulation. We theoretically estimated the resolution improvement and the sidelobe effect in the point spread function with various pupil designs and found that the combination of SAX and core-ring illumination can effectively enhance the spatial resolution in 3D and suppress sidelobe artifacts. The experimental demonstration shows that the proposed technique is effective for observation with a depth of 100 μm in a tissue phantom and can be applied to 3D observations of tissue samples with higher spatial resolution than conventional two-photon excitation microscopy.

AB - We demonstrated resolution improvement in two-photon excitation microscopy by combining saturated excitation (SAX) of fluorescence and pupil manipulation. We theoretically estimated the resolution improvement and the sidelobe effect in the point spread function with various pupil designs and found that the combination of SAX and core-ring illumination can effectively enhance the spatial resolution in 3D and suppress sidelobe artifacts. The experimental demonstration shows that the proposed technique is effective for observation with a depth of 100 μm in a tissue phantom and can be applied to 3D observations of tissue samples with higher spatial resolution than conventional two-photon excitation microscopy.

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JO - Optics Letters

JF - Optics Letters

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Saturated two-photon excitation fluorescence microscopy with core-ring illumination

Abstract

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