TY - JOUR
T1 - S100A4 mRNA is a diagnostic and prognostic marker in pancreatic carcinoma
AU - Ikenaga, Naoki
AU - Ohuchida, Kenoki
AU - Mizumoto, Kazuhiro
AU - Yu, Jun
AU - Fujita, Hayato
AU - Nakata, Kohei
AU - Ueda, Junji
AU - Sato, Norihiro
AU - Nagai, Eishi
AU - Tanaka, Masao
N1 - Funding Information:
Supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan and grants from the Takeda Science Foundation, Pancreas Research Foundation of Japan, and Nakajima Foundation. N.Ikenaga.K.Ohuchida(*).K.Mizumoto.J.Yu.H.Fujita. K. Nakata.J. Ueda.N. Sato.E. Nagai.M. Tanaka Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Fukuoka 812-8582, Japan e-mail: kenoki@surg1.med.kyushu-u.ac.jp
PY - 2009/9
Y1 - 2009/9
N2 - Objective The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer. Materials and Methods We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction. Results IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028). Conclusion S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.
AB - Objective The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer. Materials and Methods We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction. Results IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028). Conclusion S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.
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U2 - 10.1007/s11605-009-0978-4
DO - 10.1007/s11605-009-0978-4
M3 - Article
C2 - 19653048
AN - SCOPUS:70350409609
SN - 1091-255X
VL - 13
SP - 1852
EP - 1858
JO - Journal of Gastrointestinal Surgery
JF - Journal of Gastrointestinal Surgery
IS - 10
ER -
