TY - JOUR
T1 - Role of the Atg9a gene in intrauterine growth and survival of fetal mice
AU - Kojima, Takashi
AU - Yamada, Takahiro
AU - Akaishi, Rina
AU - Furuta, Itsuko
AU - Saitoh, Tatsuya
AU - Nakabayashi, Kazuhiko
AU - Nakayama, Keiichi I.
AU - Nakayama, Keiko
AU - Akira, Shizuo
AU - Minakami, Hisanori
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (Nos. 18791140 and 26462468 ), by grants from the Takeda Scientific Foundation , and by the JAOG Ogyaa Donation Foundation .
Publisher Copyright:
© 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Autophagy is activated by environment unfavorable for survival and requires Atg9a protein. Mice heterozygous for p57Kip2, devoid of the imprinted paternal allele (p57Kip2+/-), are known to develop hypertension during pregnancy. To determine whether fetal Atg9a is involved in the intrauterine survival and growth of fetal mice, this study was performed on Atg9a heterozygous (Atg9a+/-) pregnant mice with and without p57Kip2+/-. The pregnant mice heterozygous for both knockout alleles of Atg9a and p57Kip2 (Atg9a+/-/p57Kip2+/-), but not those heterozygous for Atg9a alone, developed hypertension during pregnancy. Placental expression of Atg9a mRNA was significantly decreased in the Atg9a-/- mice compared to Atg9a+/- or Atg9a+/+ mice. The Atg9a-/- fetal mice exhibited significantly retarded growth and were more likely to die in utero compared to Atg9a+/+ and Atg9a+/- fetal mice. Growth retardation was observed in the presence of maternal hypertension in Atg9a-/- fetal mice. These results suggest that Atg9a-/- fetal mice from pregnant dams heterozygous for both knockout alleles of Atg9a and p57Kip2 are more susceptible to hypertensive stress than fetuses with intact autophagic machinery.
AB - Autophagy is activated by environment unfavorable for survival and requires Atg9a protein. Mice heterozygous for p57Kip2, devoid of the imprinted paternal allele (p57Kip2+/-), are known to develop hypertension during pregnancy. To determine whether fetal Atg9a is involved in the intrauterine survival and growth of fetal mice, this study was performed on Atg9a heterozygous (Atg9a+/-) pregnant mice with and without p57Kip2+/-. The pregnant mice heterozygous for both knockout alleles of Atg9a and p57Kip2 (Atg9a+/-/p57Kip2+/-), but not those heterozygous for Atg9a alone, developed hypertension during pregnancy. Placental expression of Atg9a mRNA was significantly decreased in the Atg9a-/- mice compared to Atg9a+/- or Atg9a+/+ mice. The Atg9a-/- fetal mice exhibited significantly retarded growth and were more likely to die in utero compared to Atg9a+/+ and Atg9a+/- fetal mice. Growth retardation was observed in the presence of maternal hypertension in Atg9a-/- fetal mice. These results suggest that Atg9a-/- fetal mice from pregnant dams heterozygous for both knockout alleles of Atg9a and p57Kip2 are more susceptible to hypertensive stress than fetuses with intact autophagic machinery.
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U2 - 10.1016/j.repbio.2015.05.001
DO - 10.1016/j.repbio.2015.05.001
M3 - Article
C2 - 26370455
AN - SCOPUS:84941318259
SN - 1642-431X
VL - 15
SP - 131
EP - 138
JO - Reproductive Biology
JF - Reproductive Biology
IS - 3
ER -