Robust surface modification for protein separation chip using a film of a cellulose derivative

H. Okada; Noritada Kaji; M. Tokeshi; Y. Baba

Robust surface modification for protein separation chip using a film of a cellulose derivative

H. Okada, Noritada Kaji, M. Tokeshi, Y. Baba

Research output: Contribution to conference › Paper › peer-review

Abstract

We developed a new surface modification method using poly(methyl methacrylate) (PMMA) with a film of a cellulose derivative to ensure the long-term stability of the modification. For electrophoretic protein separation using PMMA chips as well as other polymeric chips, protein adsorption is so inevitable that analytical errors (band broadening, low detection sensitivity) are unavoidable. However, our new method overcame these drawbacks of polymeric materials and provided much better analytical performances (higher theoretical plates and consecutive runs in a single channel) than other reported methods of surface modification.

Original language	English
Pages	251-253
Number of pages	3
Publication status	Published - Jan 1 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Robust surface modification for protein separation chip using a film of a cellulose derivative. / Okada, H.; Kaji, Noritada; Tokeshi, M. et al.
2008. 251-253 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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N2 - We developed a new surface modification method using poly(methyl methacrylate) (PMMA) with a film of a cellulose derivative to ensure the long-term stability of the modification. For electrophoretic protein separation using PMMA chips as well as other polymeric chips, protein adsorption is so inevitable that analytical errors (band broadening, low detection sensitivity) are unavoidable. However, our new method overcame these drawbacks of polymeric materials and provided much better analytical performances (higher theoretical plates and consecutive runs in a single channel) than other reported methods of surface modification.

AB - We developed a new surface modification method using poly(methyl methacrylate) (PMMA) with a film of a cellulose derivative to ensure the long-term stability of the modification. For electrophoretic protein separation using PMMA chips as well as other polymeric chips, protein adsorption is so inevitable that analytical errors (band broadening, low detection sensitivity) are unavoidable. However, our new method overcame these drawbacks of polymeric materials and provided much better analytical performances (higher theoretical plates and consecutive runs in a single channel) than other reported methods of surface modification.

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ER -

Robust surface modification for protein separation chip using a film of a cellulose derivative

Abstract

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All Science Journal Classification (ASJC) codes

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