RNA-primed PCR

Hiroki Shibata, Tomoko Tahira, Kenshi Hayashi

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)


    We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of the target nucleotide sequence.

    Original languageEnglish
    Pages (from-to)400-403
    Number of pages4
    JournalGenome Research
    Issue number4
    Publication statusPublished - Nov 1995

    All Science Journal Classification (ASJC) codes

    • Genetics
    • Genetics(clinical)


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