Reversed micelles recognize an active protein

Masahiro Goto; Tsutomu Ono; Fumiyuki Nakashio; T. Alan Hatton

doi:10.1007/BF00158935

Reversed micelles recognize an active protein

Masahiro Goto, Tsutomu Ono, Fumiyuki Nakashio, T. Alan Hatton

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The extraction behavior of native and heated-denatured α-chymotrypsin has been investigated with two different reversed micellar systems. A large difference in the degree of extraction was observed for the native relative to the denatured α-chymotrypsin. In particular, mixed reversed micelles formulated with DOLPA (dioleyl phosphoric acid) and AOT show a high selectivity for the active α-chymotrypsin.

Original language	English
Pages (from-to)	141-144
Number of pages	4
Journal	Biotechnology Techniques
Volume	10
Issue number	3
DOIs	https://doi.org/10.1007/BF00158935
Publication status	Published - 1996

All Science Journal Classification (ASJC) codes

Biochemistry
Applied Microbiology and Biotechnology

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10.1007/BF00158935

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@article{766d6e3e1a934b299490830c490498d9,

title = "Reversed micelles recognize an active protein",

abstract = "The extraction behavior of native and heated-denatured α-chymotrypsin has been investigated with two different reversed micellar systems. A large difference in the degree of extraction was observed for the native relative to the denatured α-chymotrypsin. In particular, mixed reversed micelles formulated with DOLPA (dioleyl phosphoric acid) and AOT show a high selectivity for the active α-chymotrypsin.",

author = "Masahiro Goto and Tsutomu Ono and Fumiyuki Nakashio and Hatton, {T. Alan}",

year = "1996",

doi = "10.1007/BF00158935",

language = "English",

volume = "10",

pages = "141--144",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Springer Netherlands",

number = "3",

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TY - JOUR

T1 - Reversed micelles recognize an active protein

AU - Goto, Masahiro

AU - Ono, Tsutomu

AU - Nakashio, Fumiyuki

AU - Hatton, T. Alan

PY - 1996

Y1 - 1996

N2 - The extraction behavior of native and heated-denatured α-chymotrypsin has been investigated with two different reversed micellar systems. A large difference in the degree of extraction was observed for the native relative to the denatured α-chymotrypsin. In particular, mixed reversed micelles formulated with DOLPA (dioleyl phosphoric acid) and AOT show a high selectivity for the active α-chymotrypsin.

AB - The extraction behavior of native and heated-denatured α-chymotrypsin has been investigated with two different reversed micellar systems. A large difference in the degree of extraction was observed for the native relative to the denatured α-chymotrypsin. In particular, mixed reversed micelles formulated with DOLPA (dioleyl phosphoric acid) and AOT show a high selectivity for the active α-chymotrypsin.

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UR - http://www.scopus.com/inward/citedby.url?scp=0030025129&partnerID=8YFLogxK

U2 - 10.1007/BF00158935

DO - 10.1007/BF00158935

M3 - Article

AN - SCOPUS:0030025129

SN - 0951-208X

VL - 10

SP - 141

EP - 144

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 3

ER -

Reversed micelles recognize an active protein

Abstract

All Science Journal Classification (ASJC) codes

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