Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation

Yoshinori Kawabe; Tsutomu Naka; Hiroyuki Komatsu; Ken Ichi Nishijima; Shinji Iijima; Masamichi Kamihira

doi:10.1263/jbb.106.598

Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation

Yoshinori Kawabe, Tsutomu Naka, Hiroyuki Komatsu, Ken Ichi Nishijima, Shinji Iijima, Masamichi Kamihira

Molecular and Biochemical Systems Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.

Original language	English
Pages (from-to)	598-601
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	106
Issue number	6
DOIs	https://doi.org/10.1263/jbb.106.598
Publication status	Published - Dec 2008

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1263/jbb.106.598

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abstract = "A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.",

author = "Yoshinori Kawabe and Tsutomu Naka and Hiroyuki Komatsu and Nishijima, {Ken Ichi} and Shinji Iijima and Masamichi Kamihira",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research (nos. 17360396 and 20360376) from the Japan Society for the Promotion of Science (JSPS) and Technology Incubation Program in Advanced Region by the Japan Science and Technology Agency (JST).",

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AU - Kawabe, Yoshinori

AU - Naka, Tsutomu

AU - Komatsu, Hiroyuki

AU - Nishijima, Ken Ichi

AU - Iijima, Shinji

AU - Kamihira, Masamichi

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research (nos. 17360396 and 20360376) from the Japan Society for the Promotion of Science (JSPS) and Technology Incubation Program in Advanced Region by the Japan Science and Technology Agency (JST).

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Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation

Abstract

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