TY - JOUR
T1 - Restriction fragment length polymorphism analysis using random chromosomal gene probes for epidemiological analysis of Campylobacter jejuni infections
AU - Fujimoto, Shuji
AU - Umene, Kenichi
AU - Saito, Mitsumasa
AU - Horikawa, Kazumi
AU - Blaser, Martin J.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - We have evaluated the ability of a new genotyping method for Campylobacter jejuni based on restriction fragment length polymorphisms using random chromosomal gene probes. DNAs from C. jejuni strains digested with each of three restriction enzymes, HhaI, HaeIII, and HpaII, were analyzed by Southern hybridization using each of two unrelated cosmid clones, P14 and P15 (respectively containing 30- and 35-kb genomic DNA fragments of C. jejuni strain OH4384). The method reported provides a stable and discriminating means for identifying C. jejuni strains and should be useful for epidemiological analyses.
AB - We have evaluated the ability of a new genotyping method for Campylobacter jejuni based on restriction fragment length polymorphisms using random chromosomal gene probes. DNAs from C. jejuni strains digested with each of three restriction enzymes, HhaI, HaeIII, and HpaII, were analyzed by Southern hybridization using each of two unrelated cosmid clones, P14 and P15 (respectively containing 30- and 35-kb genomic DNA fragments of C. jejuni strain OH4384). The method reported provides a stable and discriminating means for identifying C. jejuni strains and should be useful for epidemiological analyses.
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U2 - 10.1128/jcm.38.4.1664-1667.2000
DO - 10.1128/jcm.38.4.1664-1667.2000
M3 - Article
C2 - 10747164
AN - SCOPUS:0033996629
SN - 0095-1137
VL - 38
SP - 1664
EP - 1667
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 4
ER -