Restoration of microglial function by granulocyte-colony stimulating factor in ALS model mice

Ryo Yamasaki; Masahito Tanaka; Mami Fukunaga; Takahisa Tateishi; Hitoshi Kikuchi; Kyoko Motomura; Takuya Matsushita; Yasumasa Ohyagi; Jun ichi Kira

doi:10.1016/j.jneuroim.2010.07.002

Restoration of microglial function by granulocyte-colony stimulating factor in ALS model mice

Ryo Yamasaki, Masahito Tanaka, Mami Fukunaga, Takahisa Tateishi, Hitoshi Kikuchi, Kyoko Motomura, Takuya Matsushita, Yasumasa Ohyagi, Jun ichi Kira

Department of Neurogy

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

We studied the effects of G-CSF on microglial reactions in mutant SOD1 (mSOD1)-Tg (G93A) ALS model mice. Following hypoglossal axotomy, the numbers of neurons and microglia expressing GDNF were significantly lower in mSOD1-Tg mice than in non-transgenic (NTG) littermates. This decrease in the number of neurons after axotomy and a decrease in the number of large myelinated axons in mSOD1-Tg mice over the disease course were improved by G-CSF, which also increased microglial recruitment. Impaired migration of cultured mSOD1-Tg microglia to MCP-1 was recovered following G-CSF treatment. Restoration of microglial responses by G-CSF may contribute to its neuroprotective effects.

Original language	English
Pages (from-to)	51-62
Number of pages	12
Journal	Journal of Neuroimmunology
Volume	229
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2010.07.002
Publication status	Published - Dec 15 2010

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2010.07.002

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@article{635dd6181d2243a889d5eda450b052d4,

title = "Restoration of microglial function by granulocyte-colony stimulating factor in ALS model mice",

abstract = "We studied the effects of G-CSF on microglial reactions in mutant SOD1 (mSOD1)-Tg (G93A) ALS model mice. Following hypoglossal axotomy, the numbers of neurons and microglia expressing GDNF were significantly lower in mSOD1-Tg mice than in non-transgenic (NTG) littermates. This decrease in the number of neurons after axotomy and a decrease in the number of large myelinated axons in mSOD1-Tg mice over the disease course were improved by G-CSF, which also increased microglial recruitment. Impaired migration of cultured mSOD1-Tg microglia to MCP-1 was recovered following G-CSF treatment. Restoration of microglial responses by G-CSF may contribute to its neuroprotective effects.",

author = "Ryo Yamasaki and Masahito Tanaka and Mami Fukunaga and Takahisa Tateishi and Hitoshi Kikuchi and Kyoko Motomura and Takuya Matsushita and Yasumasa Ohyagi and Kira, {Jun ichi}",

note = "Funding Information: This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan ; the Research Committee on Neurodegenerative Diseases of the Ministry of Health, Labour and Welfare, Japan ; and the Nakabayashi Trust for ALS Research . ",

year = "2010",

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doi = "10.1016/j.jneuroim.2010.07.002",

language = "English",

volume = "229",

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T1 - Restoration of microglial function by granulocyte-colony stimulating factor in ALS model mice

AU - Yamasaki, Ryo

AU - Tanaka, Masahito

AU - Fukunaga, Mami

AU - Tateishi, Takahisa

AU - Kikuchi, Hitoshi

AU - Motomura, Kyoko

AU - Matsushita, Takuya

AU - Ohyagi, Yasumasa

AU - Kira, Jun ichi

N1 - Funding Information: This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan ; the Research Committee on Neurodegenerative Diseases of the Ministry of Health, Labour and Welfare, Japan ; and the Nakabayashi Trust for ALS Research .

PY - 2010/12/15

Y1 - 2010/12/15

N2 - We studied the effects of G-CSF on microglial reactions in mutant SOD1 (mSOD1)-Tg (G93A) ALS model mice. Following hypoglossal axotomy, the numbers of neurons and microglia expressing GDNF were significantly lower in mSOD1-Tg mice than in non-transgenic (NTG) littermates. This decrease in the number of neurons after axotomy and a decrease in the number of large myelinated axons in mSOD1-Tg mice over the disease course were improved by G-CSF, which also increased microglial recruitment. Impaired migration of cultured mSOD1-Tg microglia to MCP-1 was recovered following G-CSF treatment. Restoration of microglial responses by G-CSF may contribute to its neuroprotective effects.

AB - We studied the effects of G-CSF on microglial reactions in mutant SOD1 (mSOD1)-Tg (G93A) ALS model mice. Following hypoglossal axotomy, the numbers of neurons and microglia expressing GDNF were significantly lower in mSOD1-Tg mice than in non-transgenic (NTG) littermates. This decrease in the number of neurons after axotomy and a decrease in the number of large myelinated axons in mSOD1-Tg mice over the disease course were improved by G-CSF, which also increased microglial recruitment. Impaired migration of cultured mSOD1-Tg microglia to MCP-1 was recovered following G-CSF treatment. Restoration of microglial responses by G-CSF may contribute to its neuroprotective effects.

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JO - Journal of Neuroimmunology

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Restoration of microglial function by granulocyte-colony stimulating factor in ALS model mice

Abstract

All Science Journal Classification (ASJC) codes

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