TY - JOUR
T1 - Restoration of Ca2+-activated tension of CDTA-treated single skeletal muscle fibers by troponin C
AU - Morimoto, Sachio
AU - Fujiwara, Takashi
AU - Ohtsuki, Iwao
PY - 1988/12
Y1 - 1988/12
N2 - Single fibers from glycerinated rabbit psoas muscle were treated with a solution containing CDTA, a strong chelator of metal ions. The CDTA-treated fibers lost all of the troponin C and showed no Ca2+ tension development. The addition of troponin C restored the Ca2+ -activated tension of CDTA-treated fibers. The tension-pCa relationship in the case of the CDTA-treated fibers reconstituted with troponin C was almost the same as that in the case of the same fibers before the CDTA treatment. These results are consistent with those of the previous study on the Ca2+ -activated ATPase of CDTA-treated rabbit skeletal myofibrils (Morimoto, S.& Ohtsuki, I. (1987) J. Biochem. 101, 291-301).
AB - Single fibers from glycerinated rabbit psoas muscle were treated with a solution containing CDTA, a strong chelator of metal ions. The CDTA-treated fibers lost all of the troponin C and showed no Ca2+ tension development. The addition of troponin C restored the Ca2+ -activated tension of CDTA-treated fibers. The tension-pCa relationship in the case of the CDTA-treated fibers reconstituted with troponin C was almost the same as that in the case of the same fibers before the CDTA treatment. These results are consistent with those of the previous study on the Ca2+ -activated ATPase of CDTA-treated rabbit skeletal myofibrils (Morimoto, S.& Ohtsuki, I. (1987) J. Biochem. 101, 291-301).
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U2 - 10.1093/oxfordjournals.jbchem.a122573
DO - 10.1093/oxfordjournals.jbchem.a122573
M3 - Article
C2 - 3149638
AN - SCOPUS:0024244935
SN - 0021-924X
VL - 104
SP - 873
EP - 874
JO - Journal of biochemistry
JF - Journal of biochemistry
IS - 6
ER -