Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

Hiroaki Nagatomo; Nanami Kohri; Hiroki Akizawa; Yumi Hoshino; Nobuhiko Yamauchi; Tomohiro Kono; Masashi Takahashi; Manabu Kawahara

doi:10.1111/asj.12538

Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

Hiroaki Nagatomo, Nanami Kohri, Hiroki Akizawa, Yumi Hoshino, Nobuhiko Yamauchi, Tomohiro Kono, Masashi Takahashi, Manabu Kawahara

Animal and Marine Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.

Original language	English
Pages (from-to)	457-461
Number of pages	5
Journal	Animal Science Journal
Volume	87
Issue number	3
DOIs	https://doi.org/10.1111/asj.12538
Publication status	Published - Mar 1 2016

All Science Journal Classification (ASJC) codes

Agricultural and Biological Sciences(all)

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10.1111/asj.12538

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title = "Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development",

abstract = "Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.",

author = "Hiroaki Nagatomo and Nanami Kohri and Hiroki Akizawa and Yumi Hoshino and Nobuhiko Yamauchi and Tomohiro Kono and Masashi Takahashi and Manabu Kawahara",

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T1 - Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

AU - Nagatomo, Hiroaki

AU - Kohri, Nanami

AU - Akizawa, Hiroki

AU - Hoshino, Yumi

AU - Yamauchi, Nobuhiko

AU - Kono, Tomohiro

AU - Takahashi, Masashi

AU - Kawahara, Manabu

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.

AB - Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.

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Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

Abstract

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