Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum

Ichiko Miyamoto; Jie Liu; Kuniyoshi Shimizu; Masao Sato; Akiko Kukita; Toshio Kukita; Ryuichiro Kondo

doi:10.1016/j.ejphar.2008.11.005

Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum

Ichiko Miyamoto, Jie Liu, Kuniyoshi Shimizu, Masao Sato, Akiko Kukita, Toshio Kukita, Ryuichiro Kondo

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Abstract

The preventative effects of the ethanol extracts of Ganoderma lucidum against the ovariectomized (Ovx)-induced deterioration of bone density in 11-week-old female Sprague Dawley (SD) rats were investigated. The results showed that the G. lucidum-treated Ovx rats showed improved bone density compared with the Ovx rats. We studied the effects of G. lucidum on osteoclastic differentiation using bone marrow cells and RAW 264 cell D-clone (RAW-D). Differentiation, in response to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor α (TNF-α), was inhibited by the ethanol extracts of G. lucidum and ganoderic acid DM which was isolated as one of the active compounds by bioassay-guided fractionation. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1). This suppression leads to the inhibition of dendritic cell-specific transmembrane protein (DC-STAMP) expression and reduces osteoclast fusion.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	European Journal of Pharmacology
Volume	602
Issue number	1
DOIs	https://doi.org/10.1016/j.ejphar.2008.11.005
Publication status	Published - Jan 5 2009

All Science Journal Classification (ASJC) codes

Pharmacology

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10.1016/j.ejphar.2008.11.005

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title = "Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum",

abstract = "The preventative effects of the ethanol extracts of Ganoderma lucidum against the ovariectomized (Ovx)-induced deterioration of bone density in 11-week-old female Sprague Dawley (SD) rats were investigated. The results showed that the G. lucidum-treated Ovx rats showed improved bone density compared with the Ovx rats. We studied the effects of G. lucidum on osteoclastic differentiation using bone marrow cells and RAW 264 cell D-clone (RAW-D). Differentiation, in response to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor α (TNF-α), was inhibited by the ethanol extracts of G. lucidum and ganoderic acid DM which was isolated as one of the active compounds by bioassay-guided fractionation. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1). This suppression leads to the inhibition of dendritic cell-specific transmembrane protein (DC-STAMP) expression and reduces osteoclast fusion.",

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AU - Miyamoto, Ichiko

AU - Liu, Jie

AU - Shimizu, Kuniyoshi

AU - Sato, Masao

AU - Kukita, Akiko

AU - Kukita, Toshio

AU - Kondo, Ryuichiro

PY - 2009/1/5

Y1 - 2009/1/5

N2 - The preventative effects of the ethanol extracts of Ganoderma lucidum against the ovariectomized (Ovx)-induced deterioration of bone density in 11-week-old female Sprague Dawley (SD) rats were investigated. The results showed that the G. lucidum-treated Ovx rats showed improved bone density compared with the Ovx rats. We studied the effects of G. lucidum on osteoclastic differentiation using bone marrow cells and RAW 264 cell D-clone (RAW-D). Differentiation, in response to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor α (TNF-α), was inhibited by the ethanol extracts of G. lucidum and ganoderic acid DM which was isolated as one of the active compounds by bioassay-guided fractionation. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1). This suppression leads to the inhibition of dendritic cell-specific transmembrane protein (DC-STAMP) expression and reduces osteoclast fusion.

AB - The preventative effects of the ethanol extracts of Ganoderma lucidum against the ovariectomized (Ovx)-induced deterioration of bone density in 11-week-old female Sprague Dawley (SD) rats were investigated. The results showed that the G. lucidum-treated Ovx rats showed improved bone density compared with the Ovx rats. We studied the effects of G. lucidum on osteoclastic differentiation using bone marrow cells and RAW 264 cell D-clone (RAW-D). Differentiation, in response to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor α (TNF-α), was inhibited by the ethanol extracts of G. lucidum and ganoderic acid DM which was isolated as one of the active compounds by bioassay-guided fractionation. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1). This suppression leads to the inhibition of dendritic cell-specific transmembrane protein (DC-STAMP) expression and reduces osteoclast fusion.

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Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum

Abstract

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