TY - JOUR
T1 - Region-specific changes in [3H]dizocilpine binding in diazepam- withdrawn rats
AU - Tsuda, Makoto
AU - Suzuki, Tsutomu
AU - Misawa, Miwa
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid from The Tokyo Biochemical Research Foundation and a Research Grant (8A-5) for Nervous and Mental Disorders from the Ministry of Health and Welfare to T. Suzuki. We wish to thank Ms. Sachiko Komiya and Ms. Yuko Sugano for their expert technical assistance.
PY - 1998/1/9
Y1 - 1998/1/9
N2 - In the present study, to clarify the role of the N-methyl-D-aspartate (NMDA) receptor in the appearance of diazepam withdrawal signs, the changes in [3H]dizocilpine binding in several brain regions from diazepam-withdrawn rats were investigated. Brain membranes were prepared 42-45 h after termination of diazepam treatment when maximal withdrawal signs were shown. The B(max) value for [3H]dizocilpine binding was significantly increased in cerebrocortical, but not hippocampal and cerebellar, tissues from diazepam- withdrawn rats, while the K(d) value did not change in any group. Together with our previous finding that NMDA receptor antagonists potently suppress diazepam withdrawal signs, these results suggest that the upregulation of the NMDA receptor in the cerebral cortex may play an important role in the appearance of spontaneous withdrawal signs caused by discontinuation of chronic diazepam treatment.
AB - In the present study, to clarify the role of the N-methyl-D-aspartate (NMDA) receptor in the appearance of diazepam withdrawal signs, the changes in [3H]dizocilpine binding in several brain regions from diazepam-withdrawn rats were investigated. Brain membranes were prepared 42-45 h after termination of diazepam treatment when maximal withdrawal signs were shown. The B(max) value for [3H]dizocilpine binding was significantly increased in cerebrocortical, but not hippocampal and cerebellar, tissues from diazepam- withdrawn rats, while the K(d) value did not change in any group. Together with our previous finding that NMDA receptor antagonists potently suppress diazepam withdrawal signs, these results suggest that the upregulation of the NMDA receptor in the cerebral cortex may play an important role in the appearance of spontaneous withdrawal signs caused by discontinuation of chronic diazepam treatment.
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U2 - 10.1016/S0304-3940(97)00942-7
DO - 10.1016/S0304-3940(97)00942-7
M3 - Article
C2 - 9486485
AN - SCOPUS:0032498222
SN - 0304-3940
VL - 240
SP - 113
EP - 115
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -