Reduction of acetylcholine-activated current by low concentrations of extracellular adenosine 5′-triphosphate

Ken Nakazawa; Kanako Ito; Schuichi Koizumi; Yasuo Ohno; Kazuhide Inoue

doi:10.1016/0024-3205(95)02188-O

Reduction of acetylcholine-activated current by low concentrations of extracellular adenosine 5′-triphosphate

Ken Nakazawa, Kanako Ito, Schuichi Koizumi, Yasuo Ohno, Kazuhide Inoue

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Effects of adenosine 5′-triphosphate (ATP) on ionic currents activated by acetylcholine (ACh) were investigated using rat pheochromocytoma PC12 cells and Xenopus oocytes expressing nicotinic receptors. In PC12 cells, ATP (10 nM to l μM) inhibited an inward current activated by ACh in not all but about 60% of cell batches. The ACh-activated current was also inhibited by ATP in Xenopus oocytes and, with a subunit combination of α3 plus β4, the inhibition was observed at concentration as low as 100 fM. Uridine 5′-triphosphate (UTP) induced a similar inhibition of the ACh-activated current both in PC12 cells and Xenopus oocytes. These and other sproperties suggest that the current reduction by ATP is not mediated through conventional P2-purinoceptors.

Original language	English
Pages (from-to)	PL351-PL356
Journal	Life Sciences
Volume	57
Issue number	22
DOIs	https://doi.org/10.1016/0024-3205(95)02188-O
Publication status	Published - Oct 20 1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry,Genetics and Molecular Biology

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10.1016/0024-3205(95)02188-O

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title = "Reduction of acetylcholine-activated current by low concentrations of extracellular adenosine 5′-triphosphate",

abstract = "Effects of adenosine 5′-triphosphate (ATP) on ionic currents activated by acetylcholine (ACh) were investigated using rat pheochromocytoma PC12 cells and Xenopus oocytes expressing nicotinic receptors. In PC12 cells, ATP (10 nM to l μM) inhibited an inward current activated by ACh in not all but about 60% of cell batches. The ACh-activated current was also inhibited by ATP in Xenopus oocytes and, with a subunit combination of α3 plus β4, the inhibition was observed at concentration as low as 100 fM. Uridine 5′-triphosphate (UTP) induced a similar inhibition of the ACh-activated current both in PC12 cells and Xenopus oocytes. These and other sproperties suggest that the current reduction by ATP is not mediated through conventional P2-purinoceptors.",

author = "Ken Nakazawa and Kanako Ito and Schuichi Koizumi and Yasuo Ohno and Kazuhide Inoue",

note = "Funding Information: The authorsa re grateful to Prof. T. Hashimoto and Dr. K. Takeuchi of Meiji College of Pharmacy for providing K. Ito an opportunityt o study in NIHS. We also thank T. Obama for culturing PC12 cells, and Dr. R. Ishii and M. Ikeda for cooperation in preliminary experiments. This work is partly supportedb y a grant from the Ministry of Education, Science and Culture, Japan awardedt o K. N.",

year = "1995",

month = oct,

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doi = "10.1016/0024-3205(95)02188-O",

language = "English",

volume = "57",

pages = "PL351--PL356",

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T1 - Reduction of acetylcholine-activated current by low concentrations of extracellular adenosine 5′-triphosphate

AU - Nakazawa, Ken

AU - Ito, Kanako

AU - Koizumi, Schuichi

AU - Ohno, Yasuo

AU - Inoue, Kazuhide

N1 - Funding Information: The authorsa re grateful to Prof. T. Hashimoto and Dr. K. Takeuchi of Meiji College of Pharmacy for providing K. Ito an opportunityt o study in NIHS. We also thank T. Obama for culturing PC12 cells, and Dr. R. Ishii and M. Ikeda for cooperation in preliminary experiments. This work is partly supportedb y a grant from the Ministry of Education, Science and Culture, Japan awardedt o K. N.

PY - 1995/10/20

Y1 - 1995/10/20

N2 - Effects of adenosine 5′-triphosphate (ATP) on ionic currents activated by acetylcholine (ACh) were investigated using rat pheochromocytoma PC12 cells and Xenopus oocytes expressing nicotinic receptors. In PC12 cells, ATP (10 nM to l μM) inhibited an inward current activated by ACh in not all but about 60% of cell batches. The ACh-activated current was also inhibited by ATP in Xenopus oocytes and, with a subunit combination of α3 plus β4, the inhibition was observed at concentration as low as 100 fM. Uridine 5′-triphosphate (UTP) induced a similar inhibition of the ACh-activated current both in PC12 cells and Xenopus oocytes. These and other sproperties suggest that the current reduction by ATP is not mediated through conventional P2-purinoceptors.

AB - Effects of adenosine 5′-triphosphate (ATP) on ionic currents activated by acetylcholine (ACh) were investigated using rat pheochromocytoma PC12 cells and Xenopus oocytes expressing nicotinic receptors. In PC12 cells, ATP (10 nM to l μM) inhibited an inward current activated by ACh in not all but about 60% of cell batches. The ACh-activated current was also inhibited by ATP in Xenopus oocytes and, with a subunit combination of α3 plus β4, the inhibition was observed at concentration as low as 100 fM. Uridine 5′-triphosphate (UTP) induced a similar inhibition of the ACh-activated current both in PC12 cells and Xenopus oocytes. These and other sproperties suggest that the current reduction by ATP is not mediated through conventional P2-purinoceptors.

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DO - 10.1016/0024-3205(95)02188-O

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AN - SCOPUS:0028827776

SN - 0024-3205

VL - 57

SP - PL351-PL356

JO - Life Sciences

JF - Life Sciences

IS - 22

ER -

Reduction of acetylcholine-activated current by low concentrations of extracellular adenosine 5′-triphosphate

Abstract

All Science Journal Classification (ASJC) codes

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