TY - JOUR
T1 - Recovery of a hybrid vector derived from bovine papilloma virus DNA pBR322 and the HSV tk gene by bacterial transformation with extrachromosomal DNA from transfected rodent cells
AU - Takeshi, Sekiguchi
AU - Takeharu, Nishimoto
AU - Ryosuke, Kai
AU - Mutsuo, Sekiguchi
N1 - Funding Information:
We thank Drs. P.M. Howley, F. Colbere-Garapin. C. Basilic0 and S.R. Kushner for gifts of essential materials. and Dr. Y. Sakaki for technical advices. This work was supported by Scientific and Cancer Research Grants from the Ministry of Education, Science and Culture of Japan.
PY - 1983/3
Y1 - 1983/3
N2 - A bovine papilloma virus (BPV)-pBR322-derived recombinant plasmid carrying a 2-kb fragment of Herpes simplex virus DNA containing the thymidine kinase (tk) gene is capable of transforming tk cells of mouse L and Syrian hamster BHK21 lines to tk+. In transformed cells a small proportion of the plasmid DNA is present as extrachromosomal elements while the remainder appears to be integrated into the genome. It was possible to recover plasmids indistinguishable from the input DNA by transformation of Escherichia coli bacteria with low-molecular-weight DNA isolated from the transformed mouse and hamster cells and consisting of BPV-pBR322-HSV tk+ plasmids.
AB - A bovine papilloma virus (BPV)-pBR322-derived recombinant plasmid carrying a 2-kb fragment of Herpes simplex virus DNA containing the thymidine kinase (tk) gene is capable of transforming tk cells of mouse L and Syrian hamster BHK21 lines to tk+. In transformed cells a small proportion of the plasmid DNA is present as extrachromosomal elements while the remainder appears to be integrated into the genome. It was possible to recover plasmids indistinguishable from the input DNA by transformation of Escherichia coli bacteria with low-molecular-weight DNA isolated from the transformed mouse and hamster cells and consisting of BPV-pBR322-HSV tk+ plasmids.
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U2 - 10.1016/0378-1119(83)90010-0
DO - 10.1016/0378-1119(83)90010-0
M3 - Article
C2 - 6303907
AN - SCOPUS:0020540248
SN - 0378-1119
VL - 21
SP - 267
EP - 272
JO - Gene
JF - Gene
IS - 3
ER -