Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer

Pamela S. Ohashi; Tak W. Mak; Peter Van Den Elsen; Yusuke Yanagi; Yasunobu Yoshikai; Andrew F. Calman; Cox Terhorst; John D. Stobo; Arthur Weiss

doi:10.1038/316606a0

Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer

Pamela S. Ohashi, Tak W. Mak, Peter Van Den Elsen, Yusuke Yanagi, Yasunobu Yoshikai, Andrew F. Calman, Cox Terhorst, John D. Stobo, Arthur Weiss

Research output: Contribution to journal › Article › peer-review

243 Citations (Scopus)

Abstract

We have introduced a full-length complementary DNA of the T-cell antigen receptor β-chain into a mutant human T-cell line that lacked a complete β-chain messenger RNA, had a diminished level of α-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected β-chain led to a normal level of α-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.

Original language	English
Pages (from-to)	606-609
Number of pages	4
Journal	Nature
Volume	316
Issue number	6029
DOIs	https://doi.org/10.1038/316606a0
Publication status	Published - 1985
Externally published	Yes

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title = "Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer",

abstract = "We have introduced a full-length complementary DNA of the T-cell antigen receptor β-chain into a mutant human T-cell line that lacked a complete β-chain messenger RNA, had a diminished level of α-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected β-chain led to a normal level of α-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.",

author = "Ohashi, {Pamela S.} and Mak, {Tak W.} and {Van Den Elsen}, Peter and Yusuke Yanagi and Yasunobu Yoshikai and Calman, {Andrew F.} and Cox Terhorst and Stobo, {John D.} and Arthur Weiss",

year = "1985",

doi = "10.1038/316606a0",

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journal = "Nature",

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T1 - Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer

AU - Ohashi, Pamela S.

AU - Mak, Tak W.

AU - Van Den Elsen, Peter

AU - Yanagi, Yusuke

AU - Yoshikai, Yasunobu

AU - Calman, Andrew F.

AU - Terhorst, Cox

AU - Stobo, John D.

AU - Weiss, Arthur

PY - 1985

Y1 - 1985

N2 - We have introduced a full-length complementary DNA of the T-cell antigen receptor β-chain into a mutant human T-cell line that lacked a complete β-chain messenger RNA, had a diminished level of α-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected β-chain led to a normal level of α-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.

AB - We have introduced a full-length complementary DNA of the T-cell antigen receptor β-chain into a mutant human T-cell line that lacked a complete β-chain messenger RNA, had a diminished level of α-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected β-chain led to a normal level of α-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.

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U2 - 10.1038/316606a0

DO - 10.1038/316606a0

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VL - 316

SP - 606

EP - 609

JO - Nature

JF - Nature

IS - 6029

ER -

Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer

Abstract

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