Recent advances in exploiting ionic liquids for biomolecules: Solubility, stability and applications

Magaret Sivapragasam, Muhammad Moniruzzaman, Masahiro Goto

    Research output: Contribution to journalReview articlepeer-review

    148 Citations (Scopus)


    The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) – potentially attractive ”green“ and ”designer“ solvents – rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications.

    Original languageEnglish
    Pages (from-to)1000-1013
    Number of pages14
    JournalBiotechnology Journal
    Issue number8
    Publication statusPublished - Aug 1 2016

    All Science Journal Classification (ASJC) codes

    • Applied Microbiology and Biotechnology
    • Molecular Medicine


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