TY - JOUR
T1 - Rearing and breeding of germ-free mice for over 1 year in a sealed positive pressure cage system
AU - Niimi, Kimie
AU - Hardy, Patrick
AU - Bilecki, Brian
AU - Takahashi, Eiki
N1 - Funding Information:
Dr. Patrick Hardy is an employee of Allentown France SARL. Mr. Brian Bilecki is an employee of Allentown, Inc. We thank Mr. Junichi Kaga, Mr. Keiichi Yamamoto, and Mr. Soichi Hamao of Animal Care, Inc. for their careful husbandry of the germ-free mice. We also appreciate Dr. Chitoshi Itakura for encouraging this study. This work was financially supported by the cross-disciplinary project of Integrated Life Science Research to Challenge Super Aging Society at RIKEN.
Publisher Copyright:
© 2019, Hokkaido University. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Germ-free mice and gnotobiotic mice have long been used as models in various research areas. Historically, they were reared and used for studies of flexible film isolators, but such isolators have several operational disadvantages. To overcome these disadvantages, a new individually ventilated cage system that promises more standardized cage conditions and reduced operational costs for maintaining germ-free or gnotobiotic mice has been developed. However, in the context of this system, only short periods (2-12 weeks) of rearing and experimental use of germ-free or gnotobiotic mice have been reported. In this report, we describe the rearing and breeding of 117 germ-free mice for over 1 year using the Sentry SPP cage, a sealed positive pressure isolation cage dedicated to gnotobiology.
AB - Germ-free mice and gnotobiotic mice have long been used as models in various research areas. Historically, they were reared and used for studies of flexible film isolators, but such isolators have several operational disadvantages. To overcome these disadvantages, a new individually ventilated cage system that promises more standardized cage conditions and reduced operational costs for maintaining germ-free or gnotobiotic mice has been developed. However, in the context of this system, only short periods (2-12 weeks) of rearing and experimental use of germ-free or gnotobiotic mice have been reported. In this report, we describe the rearing and breeding of 117 germ-free mice for over 1 year using the Sentry SPP cage, a sealed positive pressure isolation cage dedicated to gnotobiology.
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U2 - 10.14943/jjvr.67.1.119
DO - 10.14943/jjvr.67.1.119
M3 - Article
AN - SCOPUS:85064229501
SN - 0047-1917
VL - 67
SP - 119
EP - 125
JO - Japanese Journal of Veterinary Research
JF - Japanese Journal of Veterinary Research
IS - 1
ER -