Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation

Nao Suzuki, Yoshio Nakano, Akihiro Yoshida, Yoshihisa Yamashita, Yusuke Kiyoura

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems consisting of related species that are involved in the formation and etiology of biofilms.

Original languageEnglish
Pages (from-to)3827-3830
Number of pages4
JournalJournal of Clinical Microbiology
Issue number8
Publication statusPublished - Aug 2004

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)


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