We have developed a rapid method of purifying monoclonal antibody with functional magnetite particles. Protein A, which has an affinity to IgG-type antibody, was immobilized on magnetite particles. The affinity constant of protein A-magnetite to mouse IgG was 9×l06 l/mol. Using such particles, purified antibody of 73% purity was obtained with 60% recovery from mouse hybridoma culture broth. These affinity particles could be regenerated and reused. Using magnetite particles for affinity purification, total operation time was shortened to about 20 min.
All Science Journal Classification (ASJC) codes
- General Chemistry
- General Chemical Engineering