Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis

Jin Chen, Yi Ni, Chenchen Liu, Yoshinori Yamaguchi, Qinmiao Chen, Shinichi Sekine, Xifang Zhu, Xiaoming Dou

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


High-speed capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95 s via present short-end CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R2 and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098 ng/μl, respectively.

Original languageEnglish
Pages (from-to)425-430
Number of pages6
Publication statusPublished - Nov 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry


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