Rapid extraction-HPLC was performed to estimate its usefulness for presumptive identification of Burkholderia gladioli, B. glumae and B. plantarii. On HPLC chromatograms of 12 isolates of B. gladioli, a distinct peak appeared at Rt 6.2 min without exception. This peak was absent on the chromatograms for 18 isolates of B. glumae and 5 isolates of B. plantarii tested. Benchmark peak at Rt 3.2min was observed on the chromatogram of B. plantarii. Together with the direct colony TLC method, this simplified HPLC analysis will be useful for identification of phytopathogenic bacteria.
|Number of pages
|Journal of the Faculty of Agriculture, Kyushu University
|Published - Mar 1998
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science