Rapid enzyme-linked immunosorbent assay with functional magnetite particles

Masashige Shinkai, Jin Wang, Masamichi Kamihira, Masashi Iwata, Hiroyuki Honda, Takeshi Kobayashi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.

Original languageEnglish
Pages (from-to)166-168
Number of pages3
JournalJournal of Fermentation and Bioengineering
Issue number2
Publication statusPublished - 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology


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