TY - JOUR
T1 - Rapid discrimination of two cryptic species within Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) by PCR-RFLP
AU - Takano, Shun ichiro
AU - Mochizuki, Atsushi
AU - Takasu, Keiji
AU - Konishi, Kazuhiko
AU - Alouw, Jelfina C.
AU - Pandin, Donata S.
AU - Nakamura, Satoshi
N1 - Funding Information:
Acknowledgments We are grateful to Wilco Liebregts, Ketut Sumiartha, Wayan Susila, Matias Tavares, Donny Tungga, and Sri Wahyuni for their help in collecting specimens. We also thank Kayoko Furukawa for her technical assistance. This research was supported by a JIRCAS project (Development of Biological Control against Invasive Insect Pests on Coconut Trees) and a Grant-in Aid for Scientific Research (A) (no. 21255008).
PY - 2013/6
Y1 - 2013/6
N2 - Brontispa longissima (Coleoptera: Chrysomelidae) is a serious invasive pest of coconut palm (Cocos nucifera) supposedly originating from Indonesia and New Guinea. It has recently invaded Southeast and East Asia, where it has caused serious damage to coconut plants. Brontispa longissima as currently defined contains two cryptic species: we herein referred to one as the "Asian clade", which is distributed over a wide area, including Asia and the Pacific region; and we referred to the other one as the "Pacific clade", which is found in a limited area in the Pacific region. We developed a PCR-RFLP method for differentiating the two clades. Digestion of the PCR product of a 1,014-bp region within the mitochondrial cytochrome oxidase I gene (COI) with BslI, HpyCH4III, or NlaIV resulted in clade-specific patterns as estimated by the sequence data. We applied the method to specimens newly obtained from various locations to investigate the geographical distribution of B. longissima. Although B. longissima collected from Samoa in April 2003 had been placed in the Pacific clade, specimens collected from the same island in April 2010 were placed in the Asian clade, suggesting that the predominant clade may have been changing from the former to the latter. On Timor, specimens included both clades in apparently segregated habitats.
AB - Brontispa longissima (Coleoptera: Chrysomelidae) is a serious invasive pest of coconut palm (Cocos nucifera) supposedly originating from Indonesia and New Guinea. It has recently invaded Southeast and East Asia, where it has caused serious damage to coconut plants. Brontispa longissima as currently defined contains two cryptic species: we herein referred to one as the "Asian clade", which is distributed over a wide area, including Asia and the Pacific region; and we referred to the other one as the "Pacific clade", which is found in a limited area in the Pacific region. We developed a PCR-RFLP method for differentiating the two clades. Digestion of the PCR product of a 1,014-bp region within the mitochondrial cytochrome oxidase I gene (COI) with BslI, HpyCH4III, or NlaIV resulted in clade-specific patterns as estimated by the sequence data. We applied the method to specimens newly obtained from various locations to investigate the geographical distribution of B. longissima. Although B. longissima collected from Samoa in April 2003 had been placed in the Pacific clade, specimens collected from the same island in April 2010 were placed in the Asian clade, suggesting that the predominant clade may have been changing from the former to the latter. On Timor, specimens included both clades in apparently segregated habitats.
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U2 - 10.1007/s10340-012-0474-6
DO - 10.1007/s10340-012-0474-6
M3 - Article
AN - SCOPUS:84877922514
SN - 1612-4758
VL - 86
SP - 151
EP - 155
JO - Journal of Pest Science
JF - Journal of Pest Science
IS - 2
ER -