TY - JOUR
T1 - Rapid and reliable screening method for detection of 70 pesticides in whole blood by gas chromatography-mass spectrometry using a constructed calibration-locking database
AU - Kudo, Keiko
AU - Nagamatsu, Kumi
AU - Umehara, Takahiro
AU - Usumoto, Yosuke
AU - Sameshima, Naomi
AU - Tsuji, Akiko
AU - Ikeda, Noriaki
N1 - Funding Information:
We would like to thank Hayashi Pure Chemicals, Co. Ltd. for providing pesticide standards, and Nishikawa Keisoku Co. Ltd., Shinkawa Electric Co. Ltd., and Agilent Technologies Japan, Ltd. for their helpful support with this research. We also thank Miss. K. Miller (Royal English Language Centre, Fukuoka, Japan) for revising the English used in this manuscript. Part of this work was supported by a Grant-in-Aid for Scientific Research (No. 19390185 ) from the Ministry of Education, Science, Technology, Sports and Culture .
PY - 2012/3
Y1 - 2012/3
N2 - Pesticide poisoning is one of the most common causes of death by poisoning in Japan, and various kinds of pesticides including organophosphates, carbamates and pyrethroids are listed as causative substances. The purpose of our study was to develop a rapid and reliable screening method for various kinds of pesticides in whole blood by using a unique calibration-locking database and gas chromatography-mass spectrometry. A database of 70 pesticides was constructed using NAGINATA™ software with parameters such as mass spectrum, retention time and qualifier ion/target ion ratio (QT ratio) and calibration curve. Diazepam-d 5 was used as the internal standard for construction of each calibration curve within the range of 0.01-5.0μg/ml. We examined the applicability of the constructed database by analyzing whole blood samples spiked with 70 pesticides. The pesticides in blood were extracted with hexane under acidic conditions or with an enhanced polymer column (Focus™), subjected to GC-MS, and screened by the pesticides database. Among the 70 pesticides examined, 66 and 62 were successfully identified at the level of 1 and 0.1μg/ml, respectively, by hexane and 63 and 51 were identified by the Focus column without the use of standard compounds. The time required for data analysis was significantly reduced. Since the established method can produce qualitative and semi-quantitative data without the need for standard substances, this new screening method using NAGINATA™ should be useful for confirming the presence of pesticides in blood in future clinical and forensic cases.
AB - Pesticide poisoning is one of the most common causes of death by poisoning in Japan, and various kinds of pesticides including organophosphates, carbamates and pyrethroids are listed as causative substances. The purpose of our study was to develop a rapid and reliable screening method for various kinds of pesticides in whole blood by using a unique calibration-locking database and gas chromatography-mass spectrometry. A database of 70 pesticides was constructed using NAGINATA™ software with parameters such as mass spectrum, retention time and qualifier ion/target ion ratio (QT ratio) and calibration curve. Diazepam-d 5 was used as the internal standard for construction of each calibration curve within the range of 0.01-5.0μg/ml. We examined the applicability of the constructed database by analyzing whole blood samples spiked with 70 pesticides. The pesticides in blood were extracted with hexane under acidic conditions or with an enhanced polymer column (Focus™), subjected to GC-MS, and screened by the pesticides database. Among the 70 pesticides examined, 66 and 62 were successfully identified at the level of 1 and 0.1μg/ml, respectively, by hexane and 63 and 51 were identified by the Focus column without the use of standard compounds. The time required for data analysis was significantly reduced. Since the established method can produce qualitative and semi-quantitative data without the need for standard substances, this new screening method using NAGINATA™ should be useful for confirming the presence of pesticides in blood in future clinical and forensic cases.
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U2 - 10.1016/j.legalmed.2012.01.002
DO - 10.1016/j.legalmed.2012.01.002
M3 - Article
C2 - 22289625
AN - SCOPUS:84856792859
SN - 1344-6223
VL - 14
SP - 93
EP - 100
JO - Legal Medicine
JF - Legal Medicine
IS - 2
ER -