Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells

Hideya Onishi, Akifumi Kiyota, Norihiro Koya, Hiroto Tanaka, Masayo Umebayashi, Mitsuo Katano, Takashi Morisaki

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Activated lymphocytes have the ability to undergo non-directional cell movement known as random migration, although the biological role for this remains unclear. Herein, we investigated how random migration affects cytotoxicity of activated lymphocytes using time-lapse imaging analysis. The kinetics of random migration paralleled cytotoxicity in activated lymphocytes. Sphingosine-1-phosphate (S1P) and its receptor-1 (S1PR1) play an important role in lymphocyte migration. Phosphorylated FTY720 (FTYP), a structural analog of S1P, significantly inhibited random migration and cytotoxicity of activated CD3+NKG2D+ CD8+ T-lymphocytes but not CD3-NKG2D+CD56+ natural killer (NK) cells. In a mouse xenograft model, FTYP-treated activated lymphocytes exhibited lower cytotoxicity and less tumor infiltration for activated CD3+ NKG2D+ T-lymphocytes but not CD3-NKG2D+ NK cells. These results suggest that random migration contributes to the cytotoxicity of activated CD8+ T-cells but not of NK cells.

Original languageEnglish
Pages (from-to)3947-3956
Number of pages10
JournalAnticancer research
Issue number8
Publication statusPublished - Aug 1 2014

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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