TY - JOUR
T1 - Quick analysis of baicalin in Scutellariae Radix by enzyme-linked immunosorbent assay using a monoclonal antibody
AU - Kido, Katsumi
AU - Morinaga, Osamu
AU - Shoyama, Yukihiro
AU - Tanaka, Hiroyuki
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid from JSPS's Asian CORE Program, the Ministry of Education, Culture, Sports, Science and Technology of Japan, the research fund of Kyushu University Foundation.
PY - 2008/10/19
Y1 - 2008/10/19
N2 - To establish an immunoassay for baicalin (BA), a hybridoma cell line (9D6) secreting a monoclonal antibody (MAb) against BA was prepared by cell fusion with splenocytes derived from a mouse immunized with BA-bovine serum albumin (BSA) conjugate and a myeloma cell line, SP2/0-Ag14. MAb 9D6 shows specific reactivity against BA and its aglycone, baicalein, but not against other natural products. We developed an enzyme-linked immunosorbent assay (ELISA) using MAb 9D6 in a competitive manner, ranging from 200 ng/mL to 2 μg/mL. After validating the developed ELISA on the basis of intra- and inter-assays and a recovery experiment, it was found that the ELISA was not only simple, but also sufficiently reliable and accurate for quality control of Scutellariae Radix. It allowed determination of BA in complex and mixed materials, such as Kampo medicines.
AB - To establish an immunoassay for baicalin (BA), a hybridoma cell line (9D6) secreting a monoclonal antibody (MAb) against BA was prepared by cell fusion with splenocytes derived from a mouse immunized with BA-bovine serum albumin (BSA) conjugate and a myeloma cell line, SP2/0-Ag14. MAb 9D6 shows specific reactivity against BA and its aglycone, baicalein, but not against other natural products. We developed an enzyme-linked immunosorbent assay (ELISA) using MAb 9D6 in a competitive manner, ranging from 200 ng/mL to 2 μg/mL. After validating the developed ELISA on the basis of intra- and inter-assays and a recovery experiment, it was found that the ELISA was not only simple, but also sufficiently reliable and accurate for quality control of Scutellariae Radix. It allowed determination of BA in complex and mixed materials, such as Kampo medicines.
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U2 - 10.1016/j.talanta.2008.06.034
DO - 10.1016/j.talanta.2008.06.034
M3 - Article
C2 - 18804644
AN - SCOPUS:51649100981
SN - 0039-9140
VL - 77
SP - 346
EP - 350
JO - Talanta
JF - Talanta
IS - 1
ER -