@article{2f7e667da0f0482e970ff76b261aed09,
title = "Quartz-Seq: A highly reproducible and sensitive single-cell RNA sequencing method, reveals nongenetic gene-expression heterogeneity",
abstract = "Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase.",
author = "Yohei Sasagawa and Itoshi Nikaido and Tetsutaro Hayashi and Hiroki Danno and Uno, {Kenichiro D.} and Takeshi Imai and Ueda, {Hiroki R.}",
note = "Funding Information: We thank the members of the Functional Genomics Unit and Genome Resource and Analysis Unit at the RIKEN Center for Developmental Biology (RIKEN CDB), particularly Junko Sakai, Chiharu Tanegashima, and Kazu Itomi.We also thank Hitoshi Niwa (Laboratory for Pluripotent Stem Cell Studies at RIKEN CDB) for providing the ES and PrE cells. This work was supported by the Program for Innovative Cell Biology by Innovative Technology from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, the Leading Project for the Realization of Regenerative Medicine, and the Director{\textquoteright}s Fund 2011 of RIKEN CDB. The Special Postdoctoral Researchers Program from RIKEN also supported this work. Some of the calculations were performed using the RIKEN Integrated Combined Cluster (RICC) and supercomputer system in the National Institute of Genetics (NIG), Research Organization of Information and Systems (ROIS). Funding Information: We also thank Hitoshi Niwa (Laboratory for Pluripotent Stem Cell Studies at RIKEN CDB) for providing the ES and PrE cells. This work was supported by the Program for Innovative Cell Biology by Innovative Technology from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, the Leading Project for the Realization of Regenerative Medicine, and the Director{\textquoteright}s Fund 2011 of RIKEN CDB. The Special Postdoctoral Researchers Program from RIKEN also supported this work. Some of the calculations were performed using the RIKEN Integrated Combined Cluster (RICC) and supercomputer system in the National Institute of Genetics (NIG), Research Organization of Information and Systems (ROIS). Publisher Copyright: {\textcopyright} Sasagawa et al.",
year = "2013",
month = apr,
day = "17",
doi = "10.1186/gb-2013-14-4-r31",
language = "English",
volume = "14",
journal = "Genome biology",
issn = "1474-7596",
publisher = "BioMed Central",
number = "4",
}