TY - JOUR
T1 - Quantitative determination of the representative triterpenoids in the extracts of Ganoderma lucidum with different growth stages using high-performance liquid chromatography for evaluation of their 5α-reductase inhibitory properties
AU - Liu, Jie
AU - Kurashiki, Kenji
AU - Fukuta, Atsuko
AU - Kaneko, Shuhei
AU - Suimi, Yoshitaro
AU - Shimizu, Kuniyoshi
AU - Kondo, Ryuichiro
N1 - Funding Information:
The costs of publication were supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - For quantitative determination of 5 triterpenoid constituents, including one ganoderma alcohol (ganodermatriol) and four ganoderma acids (ganoderic acid TR, DM, A, and D), in the products of Ganoderma lucidum, an analytical system was developed using high-performance liquid chromatography. The mobile phase was a linear gradient of 2% AcOH/H 2O-CH 3CN, and the elution profile was monitored at 243 and 252 nm for ganoderma alcohols and acids, respectively. This system was applied to a quantitative determination of the constituents in the different stage of G. lucidum (BMC9049 strain). The analytical results indicated that the quantity and composition of these triterpenoids differed appreciably among various stages. The stage that showed the highest concentration of ganoderic acid DM and TR also showed the strongest 5α-reductase inhibitory activity. This stage (stage 5 of 6) is thus the prime stage for harvesting this strain. Further, the contents of 5α-reductase inhibitors such as ganoderic acid TR and DM in G. lucidum extracts could be a very useful indicator to assess their 5α-reductase inhibitory activity and verify their potency.
AB - For quantitative determination of 5 triterpenoid constituents, including one ganoderma alcohol (ganodermatriol) and four ganoderma acids (ganoderic acid TR, DM, A, and D), in the products of Ganoderma lucidum, an analytical system was developed using high-performance liquid chromatography. The mobile phase was a linear gradient of 2% AcOH/H 2O-CH 3CN, and the elution profile was monitored at 243 and 252 nm for ganoderma alcohols and acids, respectively. This system was applied to a quantitative determination of the constituents in the different stage of G. lucidum (BMC9049 strain). The analytical results indicated that the quantity and composition of these triterpenoids differed appreciably among various stages. The stage that showed the highest concentration of ganoderic acid DM and TR also showed the strongest 5α-reductase inhibitory activity. This stage (stage 5 of 6) is thus the prime stage for harvesting this strain. Further, the contents of 5α-reductase inhibitors such as ganoderic acid TR and DM in G. lucidum extracts could be a very useful indicator to assess their 5α-reductase inhibitory activity and verify their potency.
UR - http://www.scopus.com/inward/record.url?scp=84862804812&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862804812&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2012.01.034
DO - 10.1016/j.foodchem.2012.01.034
M3 - Article
AN - SCOPUS:84862804812
SN - 0308-8146
VL - 133
SP - 1034
EP - 1038
JO - Food Chemistry
JF - Food Chemistry
IS - 3
ER -