TY - JOUR
T1 - Quantitative analysis of digestion resistant ACE inhibitory dipeptides by small intestinal mucosa
AU - Seki, Eiji
AU - Osajima, Katsuhiro
AU - Matsufuji, Hiroshi
AU - Matsui, Toshiro
AU - Osajima, Yutaka
PY - 1996
Y1 - 1996
N2 - The short chain peptides derived from sardines were incubated for 120 min with an extract of porcine small intestinal mucosa. Digestion-resistant dipeptides found in the digest, Val-Tyr, Val-Trp, Ile-Tyr and Ile-Trp, inhibited angiotensin I converting enzyme (ACE). Amounts of the four dipeptides isolated from the digest using a Sep-pak C-18 cartridge column and three steps of high-performance liquid chromatography, were 85.88, 21.87, 10.69 and 6.51 mg/100 g peptides, respectively, and the contribution of four peptides to total ACE inhibitory activity was 10.5%. It was confirmed that ACE inhibitory dipeptides were present in the digest of the short chain peptides.
AB - The short chain peptides derived from sardines were incubated for 120 min with an extract of porcine small intestinal mucosa. Digestion-resistant dipeptides found in the digest, Val-Tyr, Val-Trp, Ile-Tyr and Ile-Trp, inhibited angiotensin I converting enzyme (ACE). Amounts of the four dipeptides isolated from the digest using a Sep-pak C-18 cartridge column and three steps of high-performance liquid chromatography, were 85.88, 21.87, 10.69 and 6.51 mg/100 g peptides, respectively, and the contribution of four peptides to total ACE inhibitory activity was 10.5%. It was confirmed that ACE inhibitory dipeptides were present in the digest of the short chain peptides.
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U2 - 10.3136/nskkk.43.967
DO - 10.3136/nskkk.43.967
M3 - Article
AN - SCOPUS:0003751486
SN - 1341-027X
VL - 43
SP - 967
EP - 969
JO - Nippon Shokuhin Kagaku Kogaku Kaishi
JF - Nippon Shokuhin Kagaku Kogaku Kaishi
IS - 8
ER -