TY - JOUR
T1 - Quantitation of tetrodotoxin in human plasma with mixed solvent and combined cartridges
AU - Ohtsuka, Yukio
AU - Takahashi, Kou
AU - Hino, Yukiko
AU - Tsuji, Akiko
AU - Kudo, Keiko
AU - Ikeda, Noriaki
PY - 2001
Y1 - 2001
N2 - In order to improve the recovery of tetrodotoxin (TTX) in human plasma, our extraction and purification procedures were further evaluated. For the effective extraction of TTX, the use of mixed solvents, methanol-acetonitrile, with the ratio of about 8:2 was recommended. For the purification of TTX by the first cartridge, 3 kinds of cartridges, Sep-Pak C 18, Sep-Pak tC 18 and PS-2 were equally useful when complete removal of protein and lipids was carried out at the extraction step. In the purification of C 9-base by the second cartridge, PS-2 gave best recovery with one-hour holding time. The established method should be useful for the trace analysis of TTX in human plasma.
AB - In order to improve the recovery of tetrodotoxin (TTX) in human plasma, our extraction and purification procedures were further evaluated. For the effective extraction of TTX, the use of mixed solvents, methanol-acetonitrile, with the ratio of about 8:2 was recommended. For the purification of TTX by the first cartridge, 3 kinds of cartridges, Sep-Pak C 18, Sep-Pak tC 18 and PS-2 were equally useful when complete removal of protein and lipids was carried out at the extraction step. In the purification of C 9-base by the second cartridge, PS-2 gave best recovery with one-hour holding time. The established method should be useful for the trace analysis of TTX in human plasma.
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M3 - Article
AN - SCOPUS:30844452697
SN - 0915-9606
VL - 19
SP - 152
EP - 153
JO - Japanese Journal of Forensic Toxicology
JF - Japanese Journal of Forensic Toxicology
IS - 2
ER -