TY - JOUR
T1 - Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture
AU - Ikeda, Hiroki
AU - Godinho-Santos, Ana
AU - Rato, Sylvie
AU - Vanwalscappel, Bénédicte
AU - Clavel, François
AU - Aihara, Kazuyuki
AU - Iwami, Shingo
AU - Mammano, Fabrizio
N1 - Funding Information:
This research is partly supported by the Kyushu University Fund (to H.I.); the Aihara Innovative Mathematical Modeling Project, the Japan Society for the Promotion of Science (JSPS) through the “Funding Program for World-Leading Innovative R & D on Science and Technology (FIRST program)”, initiated by Council for Science and Technology Policy (to K.A. and S.I.); the Commissioned Research program of the Ministry of Health, Labour and Welfare, Japan (to K.A. and S.I. H26-ShinkoJitsuyoka-General-016); Kyushu University Short-term Young Scholar Exchange Program, which is based on Japanese Ministry of Education, Culture, Sports, Science and Technology’s grant The Program for Promoting the Enhancement of Research Universities (to S.I. and F.M.); JST PRESTO program (to S.I.); JST CREST program (to S.I.); Grants-in-Aid for Young Scientists B25800092 (to S.I.) from JSPS; JSPS KAKENHI Grant Number 10192783 (to S.I.); Inamori Foundation (to S.I.); the French National Agency for Research on AIDS and Viral Hepatitis (ANRS) 11-119 and 13-123 (to F.M.); A.G.S. is a fellow of the Portuguese Foundation for Science and Technology (FCT); S.R. was a post-doctoral fellow of the ANRS; B.V. is a doctoral fellow of Sidaction.
PY - 2015/6/29
Y1 - 2015/6/29
N2 - Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.
AB - Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.
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U2 - 10.1038/srep11761
DO - 10.1038/srep11761
M3 - Article
C2 - 26119462
AN - SCOPUS:84934269592
SN - 2045-2322
VL - 5
JO - Scientific reports
JF - Scientific reports
M1 - 11761
ER -
