TY - JOUR
T1 - Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
AU - Juengsanguanpornsuk, Wipawee
AU - Yusakul, Gorawit
AU - Kitisripanya, Tharita
AU - Krittanai, Supaluk
AU - Juengwatanatrakul, Thaweesak
AU - Sakamoto, Seiichi
AU - Putalun, Waraporn
N1 - Funding Information:
This research was supported by the Royal Golden Jubilee Ph.D. program (Grant no. PHD/0071/2560), <GS1>National Research Council of Thailand, Thailand Science Research and Innovation</GS2>; the International Research Network for Functional Food Discovery & Development (FFDD) of Thailand Science Research and Innovation (IRN61W0005); Khon Kaen University; and Grant-in-Aid from Development of Safety Evaluation Method of Health Food Containing P. mirifica (19KA3003), Research on Food Safety, the Health and Labour Sciences Research Grants, the Ministry of Health, Labour and Welfare, Japan. The facilities of the research project were provided by the Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand. The authors also thank Dr. Aekkhaluck Intharuksa, Faculty of Pharmaceutical Sciences, Chiang Mai University, Thailand, for providing the stem of P. candollei.
Funding Information:
This research was supported by the Royal Golden Jubilee Ph.D. program (Grant no. PHD/0071/2560 ), National Research Council of Thailand, Thailand Science Research and Innovation; the International Research Network for Functional Food Discovery & Development (FFDD) of Thailand Science Research and Innovation ( IRN61W0005 ); Khon Kaen University; and Grant-in-Aid from Development of Safety Evaluation Method of Health Food Containing P. mirifica ( 19KA3003 ), Research on Food Safety, the Health and Labour Sciences Research Grants, the Ministry of Health, Labour and Welfare, Japan . The facilities of the research project were provided by the Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand. The authors also thank Dr. Aekkhaluck Intharuksa, Faculty of Pharmaceutical Sciences, Chiang Mai University, Thailand, for providing the stem of P. candollei.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/1/5
Y1 - 2021/1/5
N2 - Pueraria candollei is a phytoestrogen-rich herb used to treat estrogen deficiency disorders; however, quality control of P. candollei-related health products is required for consistency of clinical outcomes. Estrogenically active (+)-7-O-methylisomiroestrol could be a potential chemical marker that facilitates the prediction of the overall estrogenic activity of P. candollei. The analytical performance of ELISA using newly produced monoclonal antibodies against methylisomiroestrol was compared with HPLC analysis. The developed indirect competitive ELISA (icELISA) was highly sensitive to methylisomiroestrol for detection, with an LOQ of 2.9 ng/mL, whereas the LOQ was 1.15 μg/mL by HPLC. The results from method validation indicated acceptable precision (1.71–6.37 % and 0.13–2.40 %) and accuracy (99.23–102.54 % and 96.84–101.88 %) of the methylisomiroestrol analysis using icELISA and HPLC. These methods were effectively applied for the determination of the methylisomiroestrol content in P. candollei samples. Apart from the plant tubers, the stem was observed as a source of methylisomiroestrol. The developed ELISA was more effective than HPLC in detecting a small quantity of methylisomiroestrol in the plant samples [0.23 × 10−3% (w/w) to 0.628 × 10−3% (w/w) dry weight]. Therefore, the ELISA could be a useful tool for the standardization of P. candollei, which is the crucial step to improve the quality of plant-derived products.
AB - Pueraria candollei is a phytoestrogen-rich herb used to treat estrogen deficiency disorders; however, quality control of P. candollei-related health products is required for consistency of clinical outcomes. Estrogenically active (+)-7-O-methylisomiroestrol could be a potential chemical marker that facilitates the prediction of the overall estrogenic activity of P. candollei. The analytical performance of ELISA using newly produced monoclonal antibodies against methylisomiroestrol was compared with HPLC analysis. The developed indirect competitive ELISA (icELISA) was highly sensitive to methylisomiroestrol for detection, with an LOQ of 2.9 ng/mL, whereas the LOQ was 1.15 μg/mL by HPLC. The results from method validation indicated acceptable precision (1.71–6.37 % and 0.13–2.40 %) and accuracy (99.23–102.54 % and 96.84–101.88 %) of the methylisomiroestrol analysis using icELISA and HPLC. These methods were effectively applied for the determination of the methylisomiroestrol content in P. candollei samples. Apart from the plant tubers, the stem was observed as a source of methylisomiroestrol. The developed ELISA was more effective than HPLC in detecting a small quantity of methylisomiroestrol in the plant samples [0.23 × 10−3% (w/w) to 0.628 × 10−3% (w/w) dry weight]. Therefore, the ELISA could be a useful tool for the standardization of P. candollei, which is the crucial step to improve the quality of plant-derived products.
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U2 - 10.1016/j.jpba.2020.113674
DO - 10.1016/j.jpba.2020.113674
M3 - Article
C2 - 33120305
AN - SCOPUS:85093694414
SN - 0731-7085
VL - 192
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
M1 - 113674
ER -