Quantification of cyanamide contents in herbaceous plants

Tsunashi Kamo; Masae Sato; Kenji Kato; Syuntaro Hiradate; Eri Nakajima; Yoshiharu Fujii; Mitsuru Hirota

doi:10.1271/bbb.60171

Quantification of cyanamide contents in herbaceous plants

Tsunashi Kamo, Masae Sato, Kenji Kato, Syuntaro Hiradate, Eri Nakajima, Yoshiharu Fujii, Mitsuru Hirota

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Cyanamide (NH₂CN) is found in nature, although it has long been recognized as an industrial product. Distribution of cyanamide in the plant kingdom was investigated using a direct quantitative determination method to detect and measure cyanamide by stable isotope dilution gas chromatography-mass spectrometry (the SID-GC-MS method). The SID-GC-MS method proved to be a robust way to quantify cyanamide contents in the extracts of 101 species of herbaceous plants. The average recovery of cyanamide from all plants tested was 55:6 ± 20:3%. Vicia villosa and V. cracca contained cyanamide at 369-498 μg/gFW and 3,460-3,579 μg/gFW respectively, while the other 99 species contained no detectable cyanamide (< 1 μg/gFW). This result suggests that distribution of cyanamide in the plant kingdom is limited and uneven.

Original language	English
Pages (from-to)	2310-2312
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	70
Issue number	9
DOIs	https://doi.org/10.1271/bbb.60171
Publication status	Published - 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.60171

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title = "Quantification of cyanamide contents in herbaceous plants",

abstract = "Cyanamide (NH2CN) is found in nature, although it has long been recognized as an industrial product. Distribution of cyanamide in the plant kingdom was investigated using a direct quantitative determination method to detect and measure cyanamide by stable isotope dilution gas chromatography-mass spectrometry (the SID-GC-MS method). The SID-GC-MS method proved to be a robust way to quantify cyanamide contents in the extracts of 101 species of herbaceous plants. The average recovery of cyanamide from all plants tested was 55:6 ± 20:3%. Vicia villosa and V. cracca contained cyanamide at 369-498 μg/gFW and 3,460-3,579 μg/gFW respectively, while the other 99 species contained no detectable cyanamide (< 1 μg/gFW). This result suggests that distribution of cyanamide in the plant kingdom is limited and uneven.",

author = "Tsunashi Kamo and Masae Sato and Kenji Kato and Syuntaro Hiradate and Eri Nakajima and Yoshiharu Fujii and Mitsuru Hirota",

note = "Funding Information: The authors would like to thank Mr. Teruo Katsu-yama of Botany Group, Kanagawa Prefectural Museum of Natural History, Odawara, Japan, for identification of the plant materials. This work was supported in part by grant aid from the Japan Ministry of Education, Culture, Sports, Science and Technology (16780082, to T.K.).",

year = "2006",

doi = "10.1271/bbb.60171",

language = "English",

volume = "70",

pages = "2310--2312",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

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TY - JOUR

T1 - Quantification of cyanamide contents in herbaceous plants

AU - Kamo, Tsunashi

AU - Sato, Masae

AU - Kato, Kenji

AU - Hiradate, Syuntaro

AU - Nakajima, Eri

AU - Fujii, Yoshiharu

AU - Hirota, Mitsuru

N1 - Funding Information: The authors would like to thank Mr. Teruo Katsu-yama of Botany Group, Kanagawa Prefectural Museum of Natural History, Odawara, Japan, for identification of the plant materials. This work was supported in part by grant aid from the Japan Ministry of Education, Culture, Sports, Science and Technology (16780082, to T.K.).

PY - 2006

Y1 - 2006

N2 - Cyanamide (NH2CN) is found in nature, although it has long been recognized as an industrial product. Distribution of cyanamide in the plant kingdom was investigated using a direct quantitative determination method to detect and measure cyanamide by stable isotope dilution gas chromatography-mass spectrometry (the SID-GC-MS method). The SID-GC-MS method proved to be a robust way to quantify cyanamide contents in the extracts of 101 species of herbaceous plants. The average recovery of cyanamide from all plants tested was 55:6 ± 20:3%. Vicia villosa and V. cracca contained cyanamide at 369-498 μg/gFW and 3,460-3,579 μg/gFW respectively, while the other 99 species contained no detectable cyanamide (< 1 μg/gFW). This result suggests that distribution of cyanamide in the plant kingdom is limited and uneven.

AB - Cyanamide (NH2CN) is found in nature, although it has long been recognized as an industrial product. Distribution of cyanamide in the plant kingdom was investigated using a direct quantitative determination method to detect and measure cyanamide by stable isotope dilution gas chromatography-mass spectrometry (the SID-GC-MS method). The SID-GC-MS method proved to be a robust way to quantify cyanamide contents in the extracts of 101 species of herbaceous plants. The average recovery of cyanamide from all plants tested was 55:6 ± 20:3%. Vicia villosa and V. cracca contained cyanamide at 369-498 μg/gFW and 3,460-3,579 μg/gFW respectively, while the other 99 species contained no detectable cyanamide (< 1 μg/gFW). This result suggests that distribution of cyanamide in the plant kingdom is limited and uneven.

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JO - Bioscience, Biotechnology and Biochemistry

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Quantification of cyanamide contents in herbaceous plants

Abstract

All Science Journal Classification (ASJC) codes

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