Quantification of cyanamide contents in herbaceous plants

Tsunashi Kamo, Masae Sato, Kenji Kato, Syuntaro Hiradate, Eri Nakajima, Yoshiharu Fujii, Mitsuru Hirota

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13 Citations (Scopus)


Cyanamide (NH2CN) is found in nature, although it has long been recognized as an industrial product. Distribution of cyanamide in the plant kingdom was investigated using a direct quantitative determination method to detect and measure cyanamide by stable isotope dilution gas chromatography-mass spectrometry (the SID-GC-MS method). The SID-GC-MS method proved to be a robust way to quantify cyanamide contents in the extracts of 101 species of herbaceous plants. The average recovery of cyanamide from all plants tested was 55:6 ± 20:3%. Vicia villosa and V. cracca contained cyanamide at 369-498 μg/gFW and 3,460-3,579 μg/gFW respectively, while the other 99 species contained no detectable cyanamide (< 1 μg/gFW). This result suggests that distribution of cyanamide in the plant kingdom is limited and uneven.

Original languageEnglish
Pages (from-to)2310-2312
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number9
Publication statusPublished - 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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