Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds

Saori Shiota, Shunsuke Yamamoto, Ayane Shimomura, Akio Ojida, Takashi Nishino, Tatsuo Maruyama

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


We quantified amino groups displayed on inorganic and organic surfaces in aqueous solution using different types of cleavable fluorescent compounds and an aldehyde dye. The cleavable fluorescent compounds were designed to bind covalently to amino groups and then liberated under specific conditions. Among the investigated materials, cleavable coumarin was most appropriate for the quantification of amino groups on silica and resin surfaces. The developed method can measure small amounts (∼pmol/cm2) of amino groups on a flat polymeric surface, detecting only amino groups that are exposed to aqueous solution and available for surface immobilization of ligands and biomolecules.

Original languageEnglish
Pages (from-to)8824-8829
Number of pages6
Issue number32
Publication statusPublished - Jul 29 2015

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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